Physicochemical property consensus sequences for functional analysis, design of multivalent antigens and targeted antivirals

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• 作者：Catherine H Schein (1) (2) (3)
  David M Bowen (1) (2)
  Jessica A Lewis (4)
  Kyung Choi (1) (2)
  Aniko Paul (5)
  Gerbrand J van der Heden van Noort (6)
  Wenzhe Lu (2)
  Dmitri V Filippov (6)
  
• 刊名：BMC Bioinformatics
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：13
• 期：13-supp
• 全文大小：1707KB
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• 作者单位：Catherine H Schein (1) (2) (3)
  David M Bowen (1) (2)
  Jessica A Lewis (4)
  Kyung Choi (1) (2)
  Aniko Paul (5)
  Gerbrand J van der Heden van Noort (6)
  Wenzhe Lu (2)
  Dmitri V Filippov (6)
  
  1. Institute for Translational Sciences, Computational Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Texas, 77555鈥?857, USA
  2. Departments of Biochemistry and Molecular Biology, University of Texas Medical Branch, Texas, 77555鈥?857, USA
  3. Microbiology and Immunology, University of Texas Medical Branch, Texas, 77555鈥?857, USA
  4. Department of Pathology, University of Texas Medical Branch, Galveston, Texas, 77555鈥?857, USA
  5. Dept. of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, 11790, USA
  6. Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300, RA, Leiden, The Netherlands
  
• ISSN：1471-2105

文摘

Background Analysis of large sets of biological sequence data from related strains or organisms is complicated by superficial redundancy in the set, which may contain many members that are identical except at one or two positions. Thus a new method, based on deriving physicochemical property (PCP)-consensus sequences, was tested for its ability to generate reference sequences and distinguish functionally significant changes from background variability. Methods The PCP consensus program was used to automatically derive consensus sequences starting from sequence alignments of proteins from Flaviviruses (from the Flavitrack database) and human enteroviruses, using a five dimensional set of Eigenvectors that summarize over 200 different scalar values for the PCPs of the amino acids. A PCP-consensus protein of a Dengue virus envelope protein was produced recombinantly and tested for its ability to bind antibodies to strains using ELISA. Results PCP-consensus sequences of the flavivirus family could be used to classify them into five discrete groups and distinguish areas of the envelope proteins that correlate with host specificity and disease type. A multivalent Dengue virus antigen was designed and shown to bind antibodies against all four DENV types. A consensus enteroviral VPg protein had the same distinctive high pKa as wild type proteins and was recognized by two different polymerases. Conclusions The process for deriving PCP-consensus sequences for any group of aligned similar sequences, has been validated for sequences with up to 50% diversity. Ongoing projects have shown that the method identifies residues that significantly alter PCPs at a given position, and might thus cause changes in function or immunogenicity. Other potential applications include deriving target proteins for drug design and diagnostic kits.