Acoustic deposition with NIMS as a high-throughput enzyme activity assay

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• 作者：Matthew Greving (1)
  Xiaoliang Cheng (23)
  Wolfgang Reindl (23)
  Benjamin Bowen (2)
  Kai Deng (34)
  Katherine Louie (2)
  Michael Nyman (1)
  Joseph Cohen (1)
  Anup Singh (34)
  Blake Simmons (34)
  Paul Adams (3)
  Gary Siuzdak (5)
  Trent Northen (23) TRNorthen@lbl.gov
• 关键词：Nanostructure initiator mass spectrometry – ; NIMS – ; Nimzyme – ; Enzyme assay – ; Glycoside hydrolase
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2012
• 出版时间：May 2012
• 年：2012
• 卷：403
• 期：3
• 页码：707-711
• 全文大小：231.1 KB
• 参考文献：1. Blanch H, Adams P, Andrews-Cramer K, Frommer W, Simmons B, Keasling JD (2008) ACS Chem Biol 3:17–20
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  6. Northen T, Lee J, Hoang L, Raymond J, Hwang D, Yannone S, Wong C, Siuzdak G (2008) Proc Natl Acad Sci USA 105:3678–3683
  7. Northen T, Yanes O, Northen M, Marrinucci D, Uritboonthai D, Apon J, Golledge S, Nordstrom A, Siuzdak G (2007) Nature 449:1033–1036
  8. Aerni H, Cornett D, Caprioli R (2006) Anal Chem 78:827–834
  9. Woo K, Northen T, Yanes O, Siuzdak G (2008) Nature Prot 3:1341–1349
  10. Deng K, George K, Reindl W, Keasling J, Adams P, Lee T, Singh A, Northen T (2011) Rapid Commun Mass Spectrom (in press)
• 作者单位：1. Nextval, 4186 Sorrento Valley Blvd, Suite G, San Diego, CA 92121, USA2. Department of Bioenergy/GTL and Structural Biology, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94704, USA3. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Joint BioEnergy Institute (JBEI), Berkeley, CA 94720, USA4. Biotechnology and Bioengineering and Biomass Science and Conversion, Technology Departments, Sandia National Laboratories, Livermore, CA 94551-0969, USA5. Department of Chemistry, Center for Metabolomics, The Scripps Research Institute, La Jolla, CA 92037, USA
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Mass spectrometry (MS)-based enzyme assay has been shown to be a useful tool for screening enzymatic activities from environmental samples. Recently, reported approaches for high-specificity multiplexed characterization of enzymatic activities allow for providing detailed information on the range of enzymatic products and monitoring multiple enzymatic reactions. However, the throughput has been limited by the slow liquid–liquid handling and manual analysis. This rapid communication demonstrates the integration of acoustic sample deposition with nanostructure initiator mass spectrometry (NIMS) imaging to provide reproducible measurements of multiple enzymatic reactions at a throughput that is tenfold to 100-fold faster than conventional MS-based enzyme assay. It also provides a simple means for the visualization of multiple reactions and reaction pathways.