Evidence of nitrogen fixation and growth promotion in canola (Brassica napus L.) by an endophytic diazotroph Paenibacillus polymyxa P2b-2R

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• 作者：Akshit Puri ; Kiran Preet Padda ; Chris P Chanway
• 关键词：Canola ; Biological nitrogen fixation ; Plant growth promotion ; Paenibacillus polymyxa ; Diazotroph ; Endophyte
• 刊名：Biology and Fertility of Soils
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：52
• 期：1
• 页码：119-125
• 全文大小：346 KB
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• 作者单位：Akshit Puri (1)
  Kiran Preet Padda (1)
  Chris P Chanway (1)
  
  1. Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, 3041-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Life Sciences
  Agriculture
  Soil Science and Conservation
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0789

文摘

An endophytic diazotroph, Paenibacillus polymyxa P2b-2R, previously isolated from internal tissues of lodgepole pine tree was tested for its ability to colonize, fix nitrogen, and promote growth of an important oilseed crop, canola. For this, we inoculated canola seeds with P. polymyxa P2b-2R and grew them for up to 60 days. Canola seedlings were harvested thrice, 20, 40, and 60 days after inoculation, to determine the rhizospheric and endophytic P2b-2R population (colony-forming units (cfu)) and quantify the biological nitrogen fixation (15N dilution assay) and growth promotion (height and biomass). The entire experiment was repeated to confirm the treatment effects. P2b-2R colonized canola rhizosphere with a population abundance of 1.41 × 108 cfu/g dry root in the first growth trial and 7.49 × 107 cfu/g dry root in the second growth trial. P2b-2R developed a persistent endophytic population in canola root, comprising 3.48 × 105 cfu/g fresh weight in the first growth trial and 2.36 × 103 cfu/g fresh weight in the second growth trial. In the first growth trial, P2b-2R inoculation enhanced seedling height by 25 % and biomass by 30 % after 60 days of inoculation. Similarly, in the second growth trial, P2b-2R inoculation promoted seedling height by 21 % and biomass by 24 %. P2b-2R inoculated seedlings fixed 22 and 19 % of nitrogen from the atmosphere in the first and second growth trials, respectively. These results clearly indicate that P. polymyxa P2b-2R, a lodgepole pine endophyte, is capable of fixing nitrogen and promoting growth of an important oilseed crop canola, which is an indication of its broad range host capability.