Identification, duplication, evolution and expression analyses of caleosins in Brassica plants and Arabidopsis subspecies

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• 作者：Yue Shen ; Mingzhe Liu ; Lili Wang ; Zhuowei Li…
• 关键词：Caleosin ; Brassica ; Characteristics ; Evolution ; Expression ; Function
• 刊名：Molecular Genetics and Genomics
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：291
• 期：2
• 页码：971-988
• 全文大小：2,472 KB
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• 作者单位：Yue Shen (1) (2)
  Mingzhe Liu (2)
  Lili Wang (2)
  Zhuowei Li (2)
  David C. Taylor (3)
  Zhixi Li (1)
  Meng Zhang (2)
  
  1. College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
  2. College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
  3. National Research Council of Canada, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biochemistry
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1617-4623

文摘

Caleosins are a class of Ca2+ binding proteins that appear to be ubiquitous in plants. Some of the main proteins embedded in the lipid monolayer of lipid droplets, caleosins, play critical roles in the degradation of storage lipids during germination and in lipid trafficking. Some of them have been shown to have histidine-dependent peroxygenase activity, which is believed to participate in stress responses in Arabidopsis. In the model plant Arabidopsis thaliana, caleosins have been examined extensively. However, little is known on a genome-wide scale about these proteins in other members of the Brassicaceae. In this study, 51 caleosins in Brassica plants and Arabidopsis lyrata were investigated and analyzed in silico. Among them, 31 caleosins, including 7 in A. lyrata, 11 in Brassica oleracea and 13 in Brassica napus, are herein identified for the first time. Segmental duplication was the main form of gene expansion. Alignment, motif and phylogenetic analyses showed that Brassica caleosins belong to either the H-family or the L-family with different motif structures and physicochemical properties. Our findings strongly suggest that L-caleosins are evolved from H-caleosins. Predicted phosphorylation sites were differentially conserved in H-caleosin and L-caleosins, respectively. ‘RY-repeat’ elements and phytohormone-related cis-elements were identified in different caleosins, which suggest diverse physiological functions. Gene structure analysis indicated that most caleosins (38 out of 44) contained six exons and five introns and their intron phases were highly conserved. Structurally integrated caleosins, such as BrCLO3-3 and BrCLO4-2, showed high expression levels and may have important roles. Some caleosins, such as BrCLO2 and BoCLO8-2, lost motifs of the calcium binding domain, proline knot, potential phosphorylation sites and haem-binding sites. Combined with their low expression, it is suggested that these caleosins may have lost function.