Comparative Estimate of the sheep breed gene pools using ISSR-analysis
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  • 作者:L. V. Nesteruk ; N. N. Makarova ; A. N. Evsyukov…
  • 关键词:genetic structure ; sheep breeds ; ISSS ; PCR ; genetic diversity
  • 刊名:Russian Journal of Genetics
  • 出版年:2016
  • 出版时间:March 2016
  • 年:2016
  • 卷:52
  • 期:3
  • 页码:304-313
  • 全文大小:1,064 KB
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  • 作者单位:L. V. Nesteruk (1)
    N. N. Makarova (2)
    A. N. Evsyukov (1)
    G. R. Svishcheva (1) (3)
    B. B. Lhasaranov (4)
    Yu. A. Stolpovsky (1)

    1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia
    2. OOO AgriVolga, Uglich, 152615, Russia
    3. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia
    4. Shuluuma IAPC, Kizhinga, 671450, Buryatia, Russia
  • 刊物类别:Biomedical and Life Sciences
  • 刊物主题:Biomedicine
    Human Genetics
    Animal Genetics and Genomics
    Microbial Genetics and Genomics
    Russian Library of Science
  • 出版者:MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
  • ISSN:1608-3369
文摘
Using the ISSR-PCR technique, the genetic structure of nine sheep breeds (Ovis aries) bred on the territories of Russia and Mongolia was examined. Species-specific and breed-specific DNA fragments were identified. For the first time, data on the genetic diversity of Telengit and Buubey sheep breeds were obtained. The main parameters of the genetic diversity and the breed structure were assessed, and the phylogenetic relationships and genetic distances between the studied breeds were determined. Using the method of hierarchical frequency averaging, the prototypal sheep gene pool was reconstructed. The three-tiered analysis of diversity based on the ISSR fingerprinting data showed that 15.8% of variability was found between the breeds, 31.4% of variability was found between the populations within the breeds, and the diversity among the individuals within the populations constituted 52.8%.

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