Biochemical Characterization of a Caspase-3 Far-red Fluorescent Probe for Non-invasive Optical Imaging of Neuronal Apoptosis

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• 作者：Valérie Jolivel (1) (2) (3) (4)
  Sébastien Arthaud (5)
  Béatrice Botia (1) (2) (4)
  Christophe Portal (3) (6)
  Bruno Delest (3)
  Guillaume Clavé (3) (6)
  Jér?me Leprince (1) (2) (4) (5)
  Anthony Romieu (6) (7)
  Pierre-Yves Renard (6) (7)
  Omar Touzani (8)
  Heidi Ligeret (3)
  Pauline Noack (3)
  Marc Massonneau (3)
  Alain Fournier (4) (9)
  Hubert Vaudry (1) (2) (4) (5)
  David Vaudry (1) (2) (4) (5)
  
• 关键词：Apoptosis ; Caspase ; 3 ; Stroke ; Imaging
• 刊名：Journal of Molecular Neuroscience
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：54
• 期：3
• 页码：451-462
• 全文大小：1,025 KB
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• 作者单位：Valérie Jolivel (1) (2) (3) (4)
  Sébastien Arthaud (5)
  Béatrice Botia (1) (2) (4)
  Christophe Portal (3) (6)
  Bruno Delest (3)
  Guillaume Clavé (3) (6)
  Jér?me Leprince (1) (2) (4) (5)
  Anthony Romieu (6) (7)
  Pierre-Yves Renard (6) (7)
  Omar Touzani (8)
  Heidi Ligeret (3)
  Pauline Noack (3)
  Marc Massonneau (3)
  Alain Fournier (4) (9)
  Hubert Vaudry (1) (2) (4) (5)
  David Vaudry (1) (2) (4) (5)
  
  1. Rouen Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen, 76821, Mont-Saint-Aignan, France
  2. INSERM U982, DC2N, University of Rouen, 76821, Mont-Saint-Aignan, France
  3. QUIDD, Pharmaparc II, Voie de l’Innovation, 27100, Val de Reuil, France
  4. International Associated Laboratory Samuel de Champlain http://www.recherche-technologie-hn.com/en/champlain.php
  5. Cell Imaging Platform of Normandy (PRIMACEN), University of Rouen, 76821, Mont-Saint-Aignan, France
  6. Equipe de chimie Bio-Organique, COBRA CNRS UMR 6014 & FR 3038, IRCOF, 76131, Mont-Saint-Aignan, France
  7. University of Rouen, IRCOF, rue Tesnière, 76821, Mont-Saint-Aignan, France
  8. CERVOxy team, CNRS UMR 6232, Cycéron, 14074, Caen, France
  9. INRS–Institut Armand-Frappier, University of Québec, 531 Boulevard des Prairies, Laval, Québec, H7V1B7, Canada
  
• ISSN：1559-1166

文摘

Apoptosis is a regulated process, leading to cell death, which is involved in several pathologies including neurodegenerative diseases and stroke. Caspase-3 is a key enzyme of the apoptotic pathway and is considered as a major target for the treatment of abnormal cell death. Sensitive and non-invasive methods to monitor caspase-3 activity in cells and in the brain of living animals are needed to test the efficiency of novel therapeutic strategies. In the present study, we have biochemically characterized a caspase-3 far-red fluorescent probe, QCASP3.2, that can be used to detect apoptosis in vivo. The specificity of cleavage of QCASP3.2 was demonstrated using recombinant caspases and protease inhibitors. The functionality of the probe was also established in cerebellar neurons cultured in apoptotic conditions. QCASP3.2 did not exhibit any toxicity and appeared to accurately reflect the induction and inhibition of caspase activity by H2O2 and PACAP, respectively, both in cell lysates and in cultured neurons. Finally, intravenous injection of the probe after cerebral ischemia revealed activation of caspase-3 in the infarcted hemisphere. Thus, the present study demonstrates that QCASP3.2 is a suitable probe to monitor apoptosis both in vitro and in vivo and illustrates some of the possible applications of this caspase-3 fluorescent probe.