First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

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• 作者：Alireza Zahedi ; Jordan Phasey ; Tony Boland ; Una Ryan
• 关键词：Cryptosporidium ; Buffalo ; 18S ; C. parvum ; C. bovis ; gp60
• 刊名：Parasitology Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：115
• 期：3
• 页码：1349-1353
• 全文大小：271 KB
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• 作者单位：Alireza Zahedi (1)
  Jordan Phasey (2)
  Tony Boland (3)
  Una Ryan (1)
  
  1. School of Veterinary and Life Sciences, Murdoch University, Perth, Australia
  2. Indigenous Essential Services, Power and Water Corporation, Darwin, NT, Australia
  3. Tropical Water Solutions Pty. Ltd., Woolner, Australia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

A molecular epidemiological survey of Cryptosporidium from water buffalo (Bubalus bubalis) in the Northern Territory in Australia was conducted. Fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18S quantitative PCR (qPCR). Positives were typed by sequence analysis of 18S nested PCR products. The qPCR prevalence of Cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. Sequence analysis identified two species: C. parvum and C. bovis, with C. parvum accounting for ~80 % of positives typed from the farmed buffalo fecal samples compared to 50 % for wild buffalo. Subtyping at the 60 kDa glycoprotein (gp60) locus identified C. parvum subtypes IIdA19G1 (n = 4) and IIdA15G1 (n = 1) in the farmed buffalo and IIaA18G3R1 (n = 2) in the wild buffalo. The presence of C. parvum, which commonly infects humans, suggests that water buffaloes may contribute to contamination of rivers and waterways with human infectious Cryptosporidium oocysts, and further research on the epidemiology of Cryptosporidium in buffalo populations in Australia is required.