Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus

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• 作者：Minmin Zhang ; Jinying Ge ; Zhiyuan Wen ; Weiye Chen ; Xijun Wang…
• 刊名：Archives of Virology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：162
• 期：2
• 页码：359-367
• 全文大小：1045KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Virology; Medical Microbiology; Infectious Diseases;
• 出版者：Springer Vienna
• ISSN：1432-8798
• 卷排序：162

文摘

Bovine ephemeral fever (BEF) is caused by the arthropod-borne bovine ephemeral fever virus (BEFV), which is a member of the family Rhabdoviridae and the genus Ephemerovirus. BEFV causes an acute febrile infection in cattle and water buffalo. In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of BEFV (rL-BEFV-G) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. BEFV G enabled NDV to spread from cell to cell. rL-BEFV-G remained nonvirulent in poultry and mice compared with vector LaSota virus. rL-BEFV-G triggered a high titer of neutralizing antibodies against BEFV in mice and cattle. These results suggest that rL-BEFV-G might be a suitable candidate vaccine against BEF.