Specific Oligonucleotide Primers Based on Sequences of the 16S-23S rDNA Spacer Region for the Detection of Burkholderia gladioli by PCR
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- 作者：Naruto FURUYA ; Hiroyuki URA ; Kazuhiro IIYAMA ; Masaru MATSUMOTO ; Minoru TAKESHITA and Yoichi TAKANAMI
- 关键词：Key words ; Burkholderia gladioli ; Burkholderia glumae ; rDNA ; PCR.
- 刊名：Journal of General Plant Pathology
- 出版年：2002
- 出版时间：August 2002
- 年：2002
- 卷：68
- 期：3
- 页码：220-224
- 全文大小：1,096 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Pathology
Agriculture
Microbiology - 出版者：Springer Japan
- ISSN：1610-739X
文摘
Specific primers were designed based on the sequences of the spacer region between the 16S and 23S ribosomal DNA (rDNA) for direct, rapid and specific detection of Burkholderia gladioli. These primers were named GLA-f and GLA-r. PCR performed on boiled bacterial suspensions yielded an amplification product of approximately 300 bp. No products from other bacterial species, including B. glumae were amplified, even after complete DNA extraction by the cetyltrimethyl-ammonium bromide (CTAB) method. Using the specific primers designed in this study, the PCR method can detect B. gladioli in plant samples within 6 hr. These data demonstrate the potential of specific PCR for the detection of B. gladioli.