Combination of a simple differential medium and toxA-specific PCR for isolation and identification of phytopathogenic Burkholderia gladioli

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• 作者：Yung-An Lee (1)
  Chin-Sheng Chao (2)
  Chi-Hung Jung (1)
  
• 关键词：Pectobacterium chrysanthemi ; Burkholderia glumae ; Phalaenopsis ; Oncidium ; Leaf rot ; Toxoflavin
• 刊名：European Journal of Plant Pathology
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：136
• 期：3
• 页码：523-533
• 全文大小：404KB
• 参考文献：1. Balandreau, J., Viallard, V., Cournoyer, B., & Coenye, T. (2001). / Burkholderia cepacia genomovar III is a common plant-associated bacterium. / Applied and Environmental Microbiology, 67, 982鈥?85. CrossRef
  2. Baxter, I. A., Lambert, P. A., & Simpson, I. N. (1997). Isolation from clinical sources of / Burkholderia cepacia possessing characteristics of / Burkholderia gladioli. / Journal of Antimicrobial Chemotherapy, 39, 169鈥?75. CrossRef
  3. Christenson, J. C., Welch, D., Mukwaya, G., Muszynski, M. J., Weaver, R. E., & Brenner, D. J. (1989). Recovery of / Pseudomonas gladioli from respiratory tract specimens of patients with cystic fibrosis. / Journal of Clinical Microbiology, 27, 270鈥?73.
  4. Chuenchitt, S., Dhirabhava, W., Karnjanarat, S., Buangsuwon, D., & Uematsu, T. (1983). A new bacterial disease on orchids / Dendrobium sp. caused by / Pseudomonas gladioli. / Kasetsart Journal, 17, 26鈥?6.
  5. Clode, F. E., Kaufmann, M. E., Malnick, H., & Pitt, T. L. (1999). Evaluation of three oligonucleotide primer sets in PCR for the identification of / Burkholderia cepacia and their differentiation from / Burkholderia gladioli. / Journal of Clinical Pathology, 52, 173鈥?76. CrossRef
  6. Coenye, T., & Vandamme, P. (2003). Diversity and significance of / Burkholderia species occupying diverse ecological niches. / Environmental Microbiology, 5, 719鈥?29. CrossRef
  7. Coenye, T., Gillis, M., & Vandamme, P. (2000). / Pseudomonas antimicrobica Attafuah and Bradbury 1990 is a junior synonym of / Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993. / International Journal of Systematic and Evolutionary Microbiology, 50, 2135鈥?139. CrossRef
  8. Furuya, N., Ura, H., Iiyama, K., Matsumoto, M., Takeshita, M., & Takanami, Y. (2002). Specific oligonucleotide primers based on sequences of the 16S鈥?3S rDNA spacer region for the detection of / Burkholderia gladioli by PCR. / Journal of General Plant Pathology, 68, 220鈥?24. CrossRef
  9. Govan, J. R. W., & Deretic, V. (1996). Microbial pathogenesis in cystic fibrosis: mucoid / Pseudomonas aerugionosa and / Burkholderia cepacia. / Microbiological Reviews, 60, 539鈥?74.
  10. Iiyama, K., Furuya, N., Takanami, Y., & Matsuyama, N. (1995). A role of phytotoxin in virulence of / Pseudomonas glumae Kurita et Tabei. / Annals of the Phytopathological Society of Japan, 61, 470鈥?76. CrossRef
  11. Jeong, Y., Kim, J., Kim, S., Kang, Y., Nagamatsu, T., & Hwang, I. (2003). Toxoflavin produced by / Burkholderia glumae causing rice grain rot is responsible for inducing bacterial wilt in many field crops. / Plant Disease, 87, 890鈥?95. CrossRef
  12. Kawaradani, M., Okada, K., & Kusakari, S. (2000). New selective medium for isolation of / Burkholderia glumae from rice seeds. / Journal of General Plant Pathology, 66, 234鈥?37. CrossRef
  13. Keith, L., Sewake, K. T., & Zee, F. T. (2005). Isolation and characterization of / Burkholderia gladioli from orchids in Hawaii. / Plant Disease, 89, 1273鈥?278. CrossRef
  14. Lee, Y.-A., & Chan, C.-W. (2007). Molecular typing and presence of genetic markers among strains of banana finger-tip rot pathogen, / Burkholderia cenocepacia, in Taiwan. / Phytopathology, 97, 195鈥?01. CrossRef
  15. Lee, Y.-A., & Yu, C.-P. (2006). A differential medium for the isolation and rapid identification of a plant soft rot pathogen, / Erwinia chrysanthemi. / Journal of Microbiological Methods, 64, 200鈥?06. CrossRef
  16. Lee, Y.-A., Fan, S.-C., Chiu, L.-Y., & Hsia, K.-C. (2001). Isolation of an insertion sequence from / Ralstonia solanacearum race 1 and its potential use for strain characterization and detection. / Applied and Environmental Microbiology, 67, 3943鈥?950. CrossRef
  17. Lee, Y.-A., Chan, C.-W., & Chao, C.-P. (2006). Physiological and molecular characterizations of banana finger-tip rot and onion decay pathogens in Taiwan. / Plant Pathology Bulletin, 15, 117鈥?24.
  18. Mahenthiralingam, E., Baldwin, A., & Vandamme, P. (2002). / Burkholderia cepacia complex infection in patients with cystic fibrosis. / Journal of Medical Microbiology, 51, 533鈥?38.
  19. Matsuda, I., & Sato, Z. (1988). Relations between pathogenicity and pigment productivity in the causal agent of bacterial grain rot of rice. / Annals of the Phytopathological Society of Japan, 54, 378. CrossRef
  20. Nandakumar, R., Shahjahan, A. K. M., Yuan, X. L., Dickstein, E. R., Groth, D. E., Clark, C. A., et al. (2009). / Burkholderia glumae and / B. gladioli cause bacterial panicle blight in rice in the southern United States. / Plant Disease, 93, 896鈥?05. CrossRef
  21. Ross, J., Holland, S. M., Gill, V. J., DeCarlo, E. S., & Gallin, J. I. (1995). Severe / Burkholderia (Pseudomonas) gladioli infection in chronic granulomatous disease: report of two successfully treated cases. / Clinical Infectious Diseases, 21, 1291鈥?293. CrossRef
  22. Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). / Molecular cloning: A laboratory manual (2nd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory.
  23. Sato, Z., Koiso, Y., Iwasaki, S., Matsuda, I., & Shirata, A. (1989). Toxins produced by / Pseudomonas glumae. / Annals of the Phytopathological Society of Japan, 55, 353鈥?56. CrossRef
  24. Simpson, I. N., Finlay, J., Winstanley, D. J., Dewhurst, N., Nelson, J. W., Butler, S. L., et al. (1994). Multi-resistance isolates possessing characteristics of both / Burkholderia (Pseudomonas) cepacia and / Burkholderia gladioli from patients with cystic fibrosis. / Journal of Antimicrobial Chemotherapy, 34, 353鈥?61. CrossRef
  25. Suzuki, F., Sawada, H., Azegami, K., & Tsuchiya, K. (2004). Molecular characterization of the / tox operon involved in toxoflavin biosynthesis of / Burkholderia glumae. / Journal of General Plant Pathology, 70, 97鈥?07. CrossRef
  26. Takeuchi, T., Sawada, H., Suzuki, F., & Matsuda, I. (1997). Specific detection of / Burkholderia plantarii and / B. glumae by using primers selected from the 16S鈥?3S rDNA spacer regions. / Annals of the Phytopathological Society of Japan, 63, 455鈥?62. CrossRef
  27. Tsushima, S., Wakimoto, T., & Mogi, S. (1986). Selective medium for detecting / Pseudomonas glumae Kurita et Tabei, the causal bacterium of grain rot of rice. / Annals of the Phytopathological Society of Japan, 52, 253鈥?59. CrossRef
  28. Whitby, P. W., Pope, L. C., Carter, K. B., Lipuma, J. J., & Stull, T. L. (2000). Species-specific PCR as a tool for the identification of / Burkholderia gladioli. / Journal of Clinical Microbiology, 38, 282鈥?85.
  
• 作者单位：Yung-An Lee (1)
  Chin-Sheng Chao (2)
  Chi-Hung Jung (1)
  
  1. Department of Life Science, Fu Jen Catholic University, Xinzhuang Dist., New Taipei City, 24205, Taiwan, Republic of China
  2. Graduate Institute of Applied Science and Engineering, Fu Jen Catholic University, Xinzhuang Dist., New Taipei City, 24205, Taiwan, Republic of China
  
• ISSN：1573-8469

文摘

The NGM medium developed in a previous study was used for differential isolation of Pectobacterium chrysanthemi, Burkholderia gladioli, and B. glumae. P. chrysanthemi developed blue colonies, and all B. gladioli and B. glumae strains tested produced diffusible yellow pigments on the NGM medium, easily distinguishable from other Burkholderia spp. and plant pathogenic bacteria. The produced yellow pigments contained a toxoflavin determined by the TLC and orchid leaf chlorosis tests. A specific oligonucleotide primer pair was designed for the detection of toxA, which is involved in toxoflavin biosynthesis. All B. gladioli and B. glumae strains tested contained toxA as determined by PCR amplification. No amplification was observed with other plant pathogenic bacteria. In addition, the toxA-based species-specific PCR assays, based on the nucleotide sequence differences in the promoter region of toxA, were developed for identification of B. gladioli and B. glumae, respectively. The NGM medium and the toxA-based PCR assays were used to determine the causal agents of leaf rot of Phalaenopsis and Oncidium orchids at three cultivation areas in Taiwan. It was found that both P. chrysanthemi and B. gladioli are important pathogenic bacteria of orchid leaf rot in Taiwan. The results indicate that the combination of NGM medium with toxA-based PCR assays is a newly designed and efficient method for isolation and identification of leaf rot pathogenic bacteria especially from plant hosts on which P. chrysanthemi and B. gladioli (or B. glumae) could cause symptoms.