Effect of human recombinant granulocyte colony-stimulating factor on rat busulfan-induced testis injury

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• 作者：Neda Khanlarkhani ; Parichehr Pasbakhsh ; Keywan Mortezaee…
• 关键词：Busulfan ; Granulocyte colony ; stimulating factor ; Infertility ; Spermatogenesis
• 刊名：Journal of Molecular Histology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：47
• 期：1
• 页码：59-67
• 全文大小：5,437 KB
• 参考文献：Anderson RA, Mitchell RT, Kelsey TW, Spears N, Telfer EE, Wallace WHB (2015) Cancer treatment and gonadal function: experimental and established strategies for fertility preservation in children and young adults. Lancet Diabetes Endocrinol 3:556–567PubMed CrossRef
  Anjamrooz SH, Movahedin M, Mowla SJ, Pour Bairanvand S (2007) Assessment of morphological and functional changes in the mouse testis and epididymal sperms following busulfan treatment. Iran Biomed J 11:15–22PubMed
  Benavides-Garcia R, Joachim R, Pina NA, Mutoji KN, Reilly MA, Hermann BP (2015) Granulocyte colony-stimulating factor prevents loss of spermatogenesis after sterilizing busulfan chemotherapy. Fertil Steril 103(270–280):e278
  Cai L et al (2015a) The effects of human recombinant granulocyte-colony stimulating factor treatment during in vitro maturation of porcine oocyte on subsequent embryonic development. Theriogenology 84:1075–1087PubMed CrossRef
  Cai Y, Liu T, Fang F, Shen S, Xiong C (2015b) Involvement of ICAM‐1 in impaired spermatogenesis after busulfan treatment in mice. Andrologia. doi:10.​1111/​and.​12414
  Choi Y-J et al (2004) Murine male germ cell apoptosis induced by busulfan treatment correlates with loss of c-kit-expression in a Fas/FasL-and p53-independent manner. FEBS Lett 575:41–51PubMed CrossRef
  Costoya JA et al (2004) Essential role of Plzf in maintenance of spermatogonial stem cells. Nat Genet 36:653–659PubMed CrossRef
  Dwarakanath M, Lim M, Xu H, Hong Y (2014) Differential expression of boule and dazl in adult germ cells of the Asian seabass. Gene 549:237–242PubMed CrossRef
  Fraczek M, Kurpisz M (2015) Cytokines in the male reproductive tract and their role in infertility disorders. J Reprod Immunol 108:98–104PubMed CrossRef
  Harada M et al (2005) G-CSF prevents cardiac remodeling after myocardial infarction by activating the Jak–Stat pathway in cardiomyocytes. Nat Med 11:305–311PubMed CrossRef
  Hermann BP (2014) Method for treating chemotherpay-induced male infertility. Google Patents
  Jackson H, Partington M, Fox B (1962) Effect of Busulphan ‘(Myleran’) on the spermatogenic cell population of the rat testis. Nature 194:1184–1185PubMed CrossRef
  Jung S-W et al. (2015) Effects of Korean Red Ginseng extract on busulfan-induced dysfunction of the male reproductive system. J Ginseng Res
  Kim J, Lee S, Jeon B, Jang W, Moon C, Kim S (2011) Protection of spermatogenesis against gamma ray-induced damage by granulocyte colony-stimulating factor in mice. Andrologia 43:87–93PubMed CrossRef
  Lai D, Wang F, Yao X, Zhang Q, Wu X, Xiang C (2015) Human endometrial mesenchymal stem cells restore ovarian function through improving the renewal of germline stem cells in a mouse model of premature ovarian failure. J Transl Med 13:1–13CrossRef
  Lan X, Qu H, Yao W, Zhang C (2008) Granulocyte-colony stimulating factor inhibits neuronal apoptosis in a rat model of diabetic cerebral ischemia. Tohoku J Exp Med 216:117–126PubMed CrossRef
  Luo Z et al (2015) microRNA profiling in three main stages during porcine spermatogenesis. J Assist Reprod Genet 32:451–460PubMed CrossRef
  Mallick C, Mandal S, Barik B, Bhattacharya A, Ghosh D (2007) Protection of testicular dysfunctions by MTEC, a formulated herbal drug, in streptozotocin induced diabetic rat. Biol Pharma Bull 30:84–90CrossRef
  Oakberg E (1975) Effects of radiation on the testis. Endocrinology 5:233–243
  Pérez-Crespo M, Pericuesta E, Pérez-Cerezales S, Arenas MI, Lobo MV, Díaz-Gil JJ, Gutierrez-Adan A (2011) Effect of liver growth factor on both testicular regeneration and recovery of spermatogenesis in busulfan-treated mice. Reprod Biol Endocrinol 9:21PubMed PubMedCentral CrossRef
  Puri P et al (2014) The transition from stem cell to progenitor spermatogonia and male fertility requires the SHP2 protein tyrosine phosphatase. Stem Cells 32:741–753PubMed CrossRef
  Samplaski MK, Dimitromanolakis A, Lo KC, Grober ED, Mullen B, Garbens A, Jarvi KA (2015) The relationship between sperm viability and DNA fragmentation rates. Reprod Biol Endocrinol 13:42PubMed PubMedCentral CrossRef
  Savadi-Shiraz E et al (2015) Quantification of sperm specific mRNA transcripts (PRM1, PRM2, and TNP2) in teratozoospermia and normozoospermia: New correlations between mRNA content and morphology of sperm. Mol Reprod Dev 82:26–35PubMed CrossRef
  Schneider A et al (2005) The hematopoietic factor G-CSF is a neuronal ligand that counteracts programmed cell death and drives neurogenesis. J Clin Investig 115:2083PubMed PubMedCentral CrossRef
  Seco‐Rovira V, Beltrán‐Frutos E, Ferrer C, Saez F, Madrid J, Canteras M, Pastor L (2015) Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster (Mesocricetus auratus) during regression owing to short photoperiod. Andrology 3:598–610PubMed CrossRef
  Shah S et al (2015) Comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (Bubalus bubalis) ovaries and testes. Reprod Domest Anim 50:365–377PubMed CrossRef
  Shim W, Mehta A, Lim SY, Zhang G, Lim CH, Chua T, Wong P (2011) G-CSF for stem cell therapy in acute myocardial infarction: friend or foe? Cardiovasc Res 89:20–30PubMed CrossRef
  Su J et al. (2015) G-CSF protects human brain vascular endothelial cells injury induced by high glucose, free fatty acids and hypoxia through MAPK and Akt signaling. PLoS ONE 10:e0120707PubMed PubMedCentral CrossRef
  Tada H, Orii H (2015) Dynamic intracellular localization of Dazl protein during Xenopus germline development. Histochem Cell Biol 144:1–10CrossRef
  Vega S, Guzman P, Garcia L, Espinosa J, de Nava CC (1988) Sperm shape abnormality and urine mutagenicity in mice treated with niclosamide. Mutat Res Genet Toxicol 204:269–276CrossRef
  Westernströer B, Terwort N, Ehmcke J, Wistuba J, Schlatt S, Neuhaus N (2014) Profiling of Cxcl12 receptors, Cxcr4 and Cxcr7 in murine testis development and a spermatogenic depletion model indicates a role for Cxcr7 in controlling Cxcl12 activity. PLoS One 9:e112598PubMed PubMedCentral CrossRef
  Zhang S et al. (2015) TNP1 functional SNPs in bta-miR-532 and bta-miR-204 target sites are associated with semen quality traits in chinese holstein bulls. Biol Reprod 92:139PubMed CrossRef
  
• 作者单位：Neda Khanlarkhani (1)
  Parichehr Pasbakhsh (1)
  Keywan Mortezaee (1)
  Mohammad Naji (1)
  Fardin Amidi (1)
  Atefeh Najafi (1)
  Aligholi Sobhani (1)
  Adib Zendedel (2)
  
  1. Department of Anatomical Sciences, Tehran University of Medical Sciences, POB 14176-1351, Tehran, Iran
  2. Institute of Neuroanatomy, RWTH Aachen University, Aachen, Germany
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Developmental Biology
  
• 出版者：Springer Netherlands
• ISSN：1567-2387

文摘

Granulocyte colony-stimulating factor (G-CSF) is known for proliferation and anti-apoptotic activities. We aimed to use this growth factor in busulfan-injured testis. 32 male Wistar rats were injected with a double dosage of 15 ml/kg busulfan with 14 days interval. Administration of human recombinant G-CSF (100 µg/kg) subcutaneously was performed in two different time periods: 3 days before and 2 days after receiving busulfan, G-CSF1; and at days 14–18 of busulfan injection, G-CSF2. Animals were sacrificed at the end of week five. Histological analysis, testis weight and sperm parameters (sperm count and viability) has been checked. Expressions of DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), deleted in azoospermia like (DAZL), transition protein 2 (TP2), proliferating cell nuclear antigen (PCNA) and 5-Bromo-20-deoxyuridine (BrdU) were assessed. Empty seminiferous tubules were apparent in the busulfan- and G-CSF2-injected rats, but not in the G-CSF1 group. The G-CSF1-treated animals showed an increase in testis weight and sperm count and viability along with high expressions of DDX4, DAZL, TP2, PCNA and BrdU; even so, the changes were reversed in the busulfan and G-CSF2 groups (for all p < 0.05). Our results revealed that G-CSF application prior to busulfan insult is a promising approach in fertility maintenance. Keywords Busulfan Granulocyte colony-stimulating factor Infertility Spermatogenesis