Effects of genetic modifications and fermentation conditions on 2,3-butanediol production by alkaliphilic Bacillus subtilis
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- 作者：Aneta M. Białkowska…
- 关键词：2 ; 3 ; Butanediol ; Fermentation ; B. subtilis ; Vitreoscilla stercoraria hemoglobin (vhb) ; Acetoin reductase/2 ; 3 ; butanediol dehydrogenase (bdhA)
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：100
- 期：6
- 页码：2663-2676
- 全文大小：975 KB
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Marzena Jędrzejczak-Krzepkowska (1)
Ewa Gromek (1)
Joanna Krysiak (1)
Barbara Sikora (1)
Halina Kalinowska (1)
Celina Kubik (1)
Fokko Schütt (2)
Marianna Turkiewicz (1)
1. Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland
2. Thünen Institute of Wood Research, Leuschnerstrasse 91, 21031, Hamburg, Germany - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
文摘
Two recombinants of alkaliphilic Bacillus subtilis LOCK 1086, constructed via different strategies such as cloning the gene encoding bacterial hemoglobin from Vitreoscilla stercoraria (vhb) and overexpression of the gene encoding acetoin reductase/2,3-butanediol dehydrogenase (bdhA) from B. subtilis LOCK 1086, did not produce more 2,3-butanediol (2,3-BD) than the parental strain. In batch fermentations, this strain synthesized 9.46 g/L in 24 h and 12.80 g/L 2,3-BD in 46 h from sugar beet molasses and an apple pomace hydrolysate, respectively. 2,3-BD production by B. subtilis LOCK 1086 was significantly enhanced in fed-batch fermentations. The highest 2,3-BD concentration (75.73 g/L in 114 h, productivity of 0.66 g/L × h) was obtained in the sugar beet molasses–based medium with four feedings with glucose. In a medium based on the apple pomace hydrolysate with three feedings with sucrose, B. subtilis LOCK 1086 produced up to 51.53 g/L 2,3-BD (in 120 h, productivity of 0.43 g/L × h).