Optimization and Application of FL-HPLC for Folates Analysis in 20 Species of Mediterranean Wild Vegetables

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• 作者：P. Morales ; V. Fernández-Ruiz ; M. C. Sánchez-Mata ; M. Cámara…
• 关键词：Folates ; HPLC ; FL ; Wild vegetables
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：8
• 期：2
• 页码：302-311
• 全文大小：832 KB
• 参考文献：1. AOAC (1993) Peer verified methods program. Manual on policies and procedures. AOAC International, Arlington
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  23. Morales P, Ferreira ICFR, Carvalho AM, Fernández-Ruiz V, Sánchez-Mata MC, Cámara M, Morales R, Tardío J (2013) Wild edibl
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

Folic acid and folates (vitamin B9) are essential for human metabolism. Their nutritional importance mainly lies in the prevention of neural tube formation defects, and other degenerative diseases. The determination of folates in food matrices is a very complex task due to the large number of chemical compounds included under the definition of folates. In the present study, an HPLC-fluorescence detection (HPLC-FL) methodology was optimized and validated to characterize total folates in vegetables. Its application to Mediterranean wild vegetables, provide new data on folates content on these species, scarcely studied so far. The highest folates contents were found in the leaves of Rumex pulcher L., Beta maritima L. and Anchusa azurea Mill. (506, 302 and 278?μg/100?g, respectively), as well as in the tender leaves and stems of Foeniculum vulgare Mill. and Silene vulgaris (Moench.) Garcke (272 and 268?μg/100?g, respectively). These data show that the studied wild vegetables are mostly a very good source of folates, compared to other folate-rich plants, such as spinach, parsley or chard. These under-utilized plants deserve attention for their potential value in current diets, as they are a good alternative for the diversification of our vegetable intake and represent an important added value as rich sources of vitamin B9.