The effects of osmolality on sperm quality in Jenynsia multidentata (Cyprinodontiformes: Anablepidae)

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• 作者：Janaína Camacho da Silva ; Antonio Sergio Varela Junior…
• 关键词：Biomarker ; Cryopreservation ; Thinner ; Sperm ; Live ; bearing ; Viviparous
• 刊名：Fish Physiology and Biochemistry
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：42
• 期：1
• 页码：93-102
• 全文大小：417 KB
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• 作者单位：Janaína Camacho da Silva (1)
  Antonio Sergio Varela Junior (1)
  Jôsie Shwartz Caldas (2)
  Clarissa da Silva Freitas (1)
  Joziel Gonçalves Botelho (1)
  Elton Pinto Colares (1)
  Carine Dahl Corcini (3) (4)
  
  1. Post-graduate in Physiological Sciences: Comparative Animal Physiology, Institute of Biological Sciences, Federal University of Rio Grande, Av. 8 km Italy, Campus Carreiros, Rio Grande, RS, 96203-900, Brazil
  2. Post-graduate in Aquatic Environments Continental Biology, Federal University of Rio Grande, Av. Italy 8 km, Rio Grande, RS, 96203-900, Brazil
  3. Department of Animal Pathology, Faculty of Veterinary Medicine, Federal University of Pelotas, University Campus, PO Box 354, Pelotas, 96001-970, Brazil
  4. Pós-Graduação em Ciências Fisiológicas: Fisiologia Animal Comparada, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Av. Itália km 8, Campus Carreiros, Rio Grande, RS, 96201-900, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Animal Physiology
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-5168

文摘

Sperm quality tests on fish are classically used for evaluating cryopreservation procedures, and they are also promising to assess aquatic toxicity and biomarkers of xenobiotic effects on reproduction. Osmotic shock from the storage medium is one of the main factors affecting sperm quality during evaluation. Thus, the objective of this study was to evaluate the effects of different osmolalities (240–460 mOsm/kg) for at least 4 days on the sperm quality parameters of the viviparous fish Jenynsia multidentata. The level of significance was (P < 0.05). The plasma osmolality of J. multidentata is 326 ± 3.9 mOsm/kg. The motility of fresh semen was higher in osmolalities of 280 and 300 mOsm/kg but did not differ between osmolalities from 240 to 320 mOsm/kg. Above 380 mOsm/kg, the motility observed was 0 %. Over the time period studied motility increased with increasing osmolality, and the most constant and long-lasting rates were between 300 and 320 mOsm/kg. On the 4th day of evaluation, higher membrane integrity rates were observed between 280 and 360 mOsm/kg, higher mitochondrial membrane potential was observed between 300 and 460 mOsm/kg, and higher DNA integrity rates were observed between 260 and 380 mOsm/kg. Moreover, osmolalities ≥460 and ≤240 resulted in the lowest motility and DNA integrity levels. Over 4 days, the plasma membrane integrity was significantly lower at ≤260 and ≥400 mOsm/kg, and the mitochondrial membrane potential was significantly lower only in osmolalities ≤240 mOsm/kg. Therefore, we conclude that for sperm quality preservation in J. multidentata, an osmolality of 300–320 mOsm/kg of the most suitable diluent is necessary. Furthermore, we conclude that the storage of sperm in a hyposmotic (<260 mOsm/kg) or hyperosmotic (>400 mOsm/kg) solution affects not only motility but also other sperm quality parameters. Keywords Biomarker Cryopreservation Thinner Sperm Live-bearing Viviparous