Long-term preservation of concentrated Nannochloropsis gaditana cultures for use in aquaculture

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• 作者：J. Camacho-Rodríguez ; M. C. Cerón-García…
• 关键词：Nannochloropsis gaditana ; Microalgae ; Storage ; Long ; term preservation ; Viability ; Fatty acids
• 刊名：Journal of Applied Phycology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：28
• 期：1
• 页码：299-312
• 全文大小：1,081 KB
• 参考文献：Alías CB, García-Malea López MC, Acién-Fernández FG, Fernández-Sevilla JM, García-Sánchez JL, Molina-Grima E (2004) Influence of power supply in the feasibility of Phaeodactylum tricornutum cultures. Biotechnol Bioeng 87:723–733CrossRef PubMed
  Aragão C, Conceição L, Dinis M, Fyhn HJ (2004) Amino acid pools of rotifers and Artemia under different conditions: nutritional implications for fish larvae. Aquaculture 234:429–445CrossRef
  Babarro JMF, Fernández-Reiriz MJ, Labarta U (2001) Influence of preservation techniques and freezing storage time on biochemical composition and spectrum of fatty acids of Isochrysis galbana clone T-ISO. Aquacult Res 32:565–572CrossRef
  Borowitzka MA (1997) Microalgae for aquaculture: opportunities and constraints. J Appl Phycol 9:393–401CrossRef
  Brown MR, Jeffrey SW, Volkman JK, Dunstan GA (1997) Nutritional properties of microalgae for mariculture. Aquaculture 151:315–331CrossRef
  Camacho-Rodríguez J, Cerón-García MC, González-López CV, Fernández-Sevilla JM, Contreras-Gómez A, Molina-Grima E (2013) A low-cost culture medium for the production of Nannochloropsis gaditana biomass optimized for aquaculture. Bioresour Technol 144:57–66CrossRef PubMed
  Cerón MC, García-Malea MC, Rivas J, Acién FG, Fernández JM, Del Río E, Guerrero MG, Molina E (2007) Antioxidant activity of Haematococcus pluvialis cells grown in continuous culture as a function of their carotenoid and fatty acid content. Appl Microbiol Biotechnol 74:1112–1119CrossRef PubMed
  Chini Zittelli G, Rodolfi L, Ponis E, Tredici MR (2003) Cold preservation and use of microalgae aquaculture feed. Abstract of the 5th European Workshop on Biotechnology of Microalgae, 23–24 June 2003, Bergholz-Rehbrücke
  Chiu SY, Kao CY, Tsai MT, Ong SC, Chen CH, Lin CS (2009) Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresour Technol 100:833–838CrossRef PubMed
  Cordero B, Voltolina D (1997) Viability of mass algal cultures preserved by freezing and freeze-drying. Aquacult Eng 16:205–211CrossRef
  Day JG, Fleck RA, Benson EE (2000) Cryopreservation-recalcitrance in microalgae: novel approaches to identify and avoid cryo-injury. J Appl Phycol 12:369–377CrossRef
  Dorsey J, Yentsch CM, Mayo S, McKenna C (1989) Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae. Cytometry 10:622–628CrossRef PubMed
  FAO Fisheries Technical Paper 361 (1996) In: Lavens P, Sorgeloos P (eds) Manual on the production and use of live food for aquaculture, 1st edn. FAO, Rome, 295 pp
  González-López CV, Cerón-García MC, Fernández-Sevilla JM, González-Céspedes AM, Camacho-Rodríguez J, Molina-Grima E (2013) Medium recycling for Nannochloropsis gaditana cultures for aquaculture. Bioresour Technol 129:430–438CrossRef PubMed
  Gouveia L, Empis J (2003) Relative stabilities of microalgal carotenoids in microalgal extracts, biomass and fish feed: effect of storage conditions. Innov Food Sci Emerg Technol 4:227–233CrossRef
  Guermazi W, Sellami-Kammoun A, Ellormi J, Drira Z, Aleya L, Marangoni R, Ayadi H, Maalej S (2010) Microalgal cryo-preservation using dimethyl sulfoxide (Me2SO) coupled with two freezing protocols: influence on the fatty acid profile. J Therm Biol 35:175–181CrossRef
  Hansmann E (1973) Pigment analysis. In: Stein JR (ed) Handbook of phycological methods, culture methods and growth measurements. Cambridge University, Cambridge, pp 359–368
  Heasman M, Diemar J, O’Connor W, Sushames T, Foulkes L (2000) Development of extended shelf-life microalgae concentrate diets harvested by centrifugation for bivalve molluscs—a summary. Aquac Res 31:637–659CrossRef
  Heras H, Kean-Howie J, Ackman RG (1994) The potential use of lipid microspheres as nutritional supplements for adult Ostrea edulis. Aquaculture 123:309–322CrossRef
  Huis in’t Veld JHJ (1996) Microbial and biochemical spoilage of foods: an overview. Int J Food Microbiol 33:1–18CrossRef
  Jones DA, Kurmaly K, Arshard A (1987) Penaeid shrimp hatchery trials using microencapsulated diets. Aquaculture 64:133–146CrossRef
  Kanazawa A, Teshima S-I, Ono K (1979) Relationship between essential fatty acid requirements of aquatic animals and the capacity for bioconversion of linolenic acid to highly unsaturated fatty acids. Comp Biochem Physiol 63B:295–298
  Molina-Grima E, Sánchez-Pérez JA, Farcía-Camacho F, Acién-Fernández FG, López-Alonso D, Segura del Castillo CI (1994) Preservation of the marine microalga, Isochrysis galbana: influence on the fatty acid profile. Aquaculture 123:377–385
  Montaini E, Chini-Zittelli G, Tredici MR, Molina-Grima E, Fernández-Sevilla JM, Sánchez-Pérez JA (1995) Long-term preservation of Tetraselmis suecica: influence of storage on viability and fatty acid profile. Aquaculture 134:81–90CrossRef
  Morais S, Koven W, Rønnestad I, Dinis MT, Conceição LEC (2005) Dietary protein: lipid ratio and lipid nature affects fatty acid absorption and metabolism in a teleost larva. Br J Nutr 93:813–820CrossRef PubMed
  Nell JA, Diemar JA, Heasman MP (1996) Food value of live yeasts and dry yeast-based diets fed to Sydney rock oyster Saccostrea commercialis spat. Aquaculture 145:235–243CrossRef
  Nunes M, Pereira A, Ferreira JF, Yasumaru F (2009) Evaluation of the microalgae paste viability produced in a mollusk hatchery in southern Brazil. J World Aquacult Soc 40:87–94CrossRef
  Parsons TR, Strickland JDH (1973) Pigment analysis. In: Stein JR (ed) Handbook of phycological methods, culture methods and growth measurements, 1st edn. London, UK, pp 359–368
  Piña P, Voltolina D, Nieves M, Robles M (2006) Survival, development and growth of the pacific white shrimp Litopenaeus vannamei protozoea larvae, fed with monoalgal and mixed diets. Aquaculture 253:523–530CrossRef
  Ponis E, Parisi G, Chini-Zittelli G, Lavista F, Robert R, Tredici MR (2008) Pavlova lutheri: production, preservation and use as food for Crassostrea gigas larvae. Aquaculture 282:97–103CrossRef
  Prado R, Rioboo C, Herrero C, Suárez-Bregua P, Cid A (2012) Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells. Ecotoxicology 21:409–420CrossRef PubMed
  Robinson CB, Samocha TM, Fox JM, Gandy RL, McKee DA (2005) The use of inert artificial commercial food sources as replacements of traditional live food items in the culture of larval shrimp, Farfantepenaeus aztecus. Aquaculture 245:135–147CrossRef
  Rocha JMS, García JEC, Henriques MHF (2003) Growth aspects of the marine microalga Nannochloropsis gaditana. Biomol Eng 20:237–242CrossRef PubMed
  Rodríguez-Ruíz J, Belarbi E, Sánchez JLG, Alonso DL (1998) Rapid simultaneous lipid extraction and transesterification for fatty acid analyses. Biotechnol Tech 12:689–691CrossRef
  Ryckebosch E, Muylaert K, Eeckhout M, Ruyssen T, Foubert I (2011) Influence of drying and storage on lipid and carotenoid stability of the microalga Phaeodactylum tricornutum. J Agric Food Chem 59:11063–11069CrossRef PubMed
  San Pedro A, González-López CV, Acién FG, Molina-Grima E (2014) Outdoor pilot-scale production of Nannochloropsis gaditana: influence of culture parameters and lipid production rates in tubular photobioreactors. Bioresour Technol 169:667–676CrossRef PubMed
  San Pedro A, González-López CV, Acién FG, Molina-Grima E (2015) Outdoor pilot production of Nannochloropsis gaditana: influence of culture parameters and lipid production rates in raceway ponds. Algal Res 8:205–213CrossRef
  Tzovenis I, Triantaphyllidis G, Naihong X, Chatzinikolaou E, Papadopoulou K, Xouri G, Tafas T (2004) Cryopreservation of marine microalgae and potential toxicity of cryoprotectants to the primary steps of the aquacultural food chain. Aquaculture 230:457–473CrossRef
  Verdelho Vieira V (2014) Microalgae for fuel and beyond: future trends. International Society for Applied Phycology Newsletter 5–17
  Vizcaíno AJ, López G, Sáez MI, Jiménez JA, Barros A, Hidalgo L, Camacho-Rodríguez J, Martínez TF, Cerón-García MC, Alarcón FJ (2014) Effects of the microalga Scenedesmus almeriensis as fishmeal alternative in diets for gilthead sea bream, Sparus aurata, juveniles. Aquaculture 431:34–43CrossRef
  Welladsen H, Kent M, Mangott A, Li Y (2014) Shelf-life assessment of microalgae concentrates: effect of cold preservation on microalgal nutrition profiles. Aquaculture 430:241–247CrossRef
  Xiao X, Han Z-Y, Chen Y-X, Liang X-Q, Li H, Qian Y-C (2011) Optimization of FDA-PI method using flow cytometry to measure metabolic activity of the cyanobacteria, Microcystis aeruginosa. Phys Chem Earth 36:424–429CrossRef
  
• 作者单位：J. Camacho-Rodríguez (1)
  M. C. Cerón-García (1)
  M. D. Macías-Sánchez (1)
  J. M. Fernández-Sevilla (1)
  L. López-Rosales (1)
  E. Molina-Grima (1)
  
  1. Chemical Engineering Area, Department of Engineering, University of Almería, 04120, Almería, Spain
  
• 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1573-5176

文摘

The aim of this paper is to solve problems that may arise in microalgae cultivation facilities at a hatchery in such a way that its activities are not disrupted. Thus, the effect of storage conditions (time, initial biomass concentration, presence or absence of light, addition of preservatives and conservation atmospheres) on cell viability, evolution of sample concentration, contamination, fatty acid and pigment content for long-term preservation of Nannochloropsis gaditana biomass has been studied. The polyunsaturated fatty acid content was maintained and the eicosapentaenoic acid (EPA, 20:5n3) content was kept stable at 3.6 % (d.w.). The total pigments content was also preserved at 0.85 % (d.w.), what allowed maintaining the antioxidant activity of samples. The results show that samples preserved both as concentrated inocula ready to run a photobioreactor, at an initial biomass (N. gaditana) concentration of 5 g L−1, and as pastes to be used as live food if any incident happens in a hatchery, at 150 g L−1, can be stored for up to 4 months at 4 °C under nitrogen or air atmosphere of conservation maintaining the cell viability and the sample concentration stable and at low incident irradiance (35 μmol photons m−2 s−1). This allows the photosynthetic activity of cells to be preserved in the case of samples at 5 g L−1. The bacterial load was kept at low level by the regular addition of preservative agents. Keywords Nannochloropsis gaditana Microalgae Storage Long-term preservation Viability Fatty acids