Gel-filtration analysis to detect Al-binding ligands in Eucalyptus camaldulensis

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• 作者：Ko Tahara ; Katsumi Kojima ; Kenji Shinohara
• 关键词：Aluminum resistance ; Citrate ; Eucalyptus camaldulensis ; Gel ; filtration chromatography
• 刊名：Journal of Forest Research
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：20
• 期：4
• 页码：411-414
• 全文大小：484 KB
• 参考文献：Brunner I, Sperisen C (2013) Aluminum exclusion and aluminum tolerance in woody plants. Front Plant Sci 4:172PubMed Central PubMed View Article
  Delhaize E, Ma JF, Ryan PR (2012) Transcriptional regulation of aluminium tolerance genes. Trends Plant Sci 17:341鈥?48PubMed View Article
  Ikka T, Ogawa T, Li DH, Hiradate S, Morita A (2013) Effect of aluminum on metabolism of organic acids and chemical forms of aluminum in root tips of Eucalyptus camaldulensis Dehnh. Phytochemistry 94:142鈥?47PubMed View Article
  Kinraide TB, Sweeney BK (2001) Buffered, phosphate-containing media suitable for aluminum toxicity studies. Plant Soil 235:75鈥?3View Article
  Kochian LV (1995) Cellular mechanisms of aluminum toxicity and resistance in plants. Annu Rev Plant Physiol Plant Mol Biol 46:237鈥?60View Article
  Kochian LV, Hoekenga OA, Pi帽eros MA (2004) How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorous efficiency. Annu Rev Plant Biol 55:459鈥?93PubMed View Article
  Ma JF (2007) Syndrome of aluminum toxicity and diversity of aluminum resistance in higher plants. Int Rev Cytol 264:225鈥?52PubMed View Article
  脰hman LO (1988) Equilibrium and structural studies of silicon(IV) and aluminum(III) in aqueous solution. 17. Stable and metastable complexes in the system H+-Al3+-citric acid. Inorg Chem 27:2565鈥?570View Article
  Osawa H, Endo I, Hara Y, Matsushima Y, Tange T (2011) Transient proliferation of proanthocyanidin-accumulating cells on the epidermal apex contributes to highly aluminum-resistant root elongation in camphor tree. Plant Physiol 155:433鈥?46PubMed Central PubMed View Article
  Ryan PR, Tyerman SD, Sasaki T, Furuichi T, Yamamoto Y, Zhang WH, Delhaize E (2011) The identification of aluminium-resistance genes provides opportunities for enhancing crop production on acid soils. J Exp Bot 62:9鈥?0PubMed View Article
  Sj枚berg S, 脰hman LO (1985) Equilibrium and structural studies of silicon(IV) and aluminium(III) in aqueous solution. Part 13. A potentiometric and 27Al nuclear magnetic resonance study of speciation and equilibria in the aluminium(III)-oxalic acid-hydroxide system. J Chem Soc Dalton Trans (12):2665鈥?669
  Tahara K, Norisada M, Hogetsu T, Kojima K (2005a) Aluminum tolerance and aluminum-induced deposition of callose and lignin in the root tips of Melaleuca and Eucalyptus species. J For Res 10:325鈥?33View Article
  Tahara K, Norisada M, Tange T, Yagi H, Kojima K (2005b) Ectomycorrhizal association enhances Al tolerance by inducing citrate secretion in Pinus densiflora. Soil Sci Plant Nutr 51:397鈥?03View Article
  Tahara K, Norisada M, Yamanoshita T, Kojima K (2008) Role of aluminum-binding ligands in aluminum resistance of Eucalyptus camaldulensis and Melaleuca cajuputi. Plant Soil 302:175鈥?87View Article
  Tahara K, Hashida K, Otsuka Y, Ohara S, Kojima K, Shinohara K (2014) Identification of a hydrolyzable tannin, oenothein B, as an aluminum-detoxifying ligand in a highly aluminum-resistant tree, Eucalyptus camaldulensis. Plant Physiol 164:683鈥?93PubMed Central PubMed View Article
  von Uexk眉ll HR, Mutert E (1995) Global extent, development and economic impact of acid soils. Plant Soil 171:1鈥?5View Article
  Wenzl P, Pati帽o GM, Chaves AL, Mayer JE, Rao IM (2001) The high level of aluminum resistance in signalgrass is not associated with known mechanisms of external aluminum detoxification in root apices. Plant Physiol 125:1473鈥?484PubMed Central PubMed View Article
  
• 作者单位：Ko Tahara (1)
  Katsumi Kojima (2)
  Kenji Shinohara (1) (3)
  
  1. Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan
  2. Asian Natural Environmental Science Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
  3. RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Forestry
  Forestry Management
  Tree Biology
  Plant Sciences
  Plant Ecology
  
• 出版者：Springer Japan
• ISSN：1610-7403

文摘

Aluminum (Al) toxicity is a major factor limiting plant growth on acid soils; inhibited root elongation is a characteristic symptom. To elucidate mechanisms of Al resistance in plants, we developed a simple method to separate Al-binding ligands from plant roots by gel-filtration chromatography. In this method, an eluent consisting of Homopipes buffer flows through a Sephadex G-25 column. Free Al ions do not pass through the column, but Al-ligand complexes can be fractionated according to differences in their molecular sizes. We applied the method to Eucalyptus camaldulensis (Myrtaceae) and successfully separated Al-binding ligands from roots. One peak of Al-ligand complex increased with Al treatment of seedlings, which can be explained by Al-induced accumulation of citrate in roots.