Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

详细信息    查看全文

• 作者：Huynh Thi Thu Hue ; Duong Thi Thu Ha…
• 关键词：4 ; coumarate ; coenzyme A ligase ; 4CL1 promoter ; Eucalyptus camaldulensis ; GUS gene ; Transgenic plant
• 刊名：Physiology and Molecular Biology of Plants
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：22
• 期：3
• 页码：399-405
• 全文大小：984 KB
• 刊物主题：Plant Sciences; Plant Physiology; Biophysics and Biological Physics; Cell Biology;
• 出版者：Springer India
• ISSN：0974-0430
• 卷排序：22

文摘

The most important enzyme of the phenylpropanoid pathway, 4-coumarate:coenzyme A ligase (4CL), is encoded by several homologous genes including 4CL1. The 4CL1 promoter is a tissue-specific gene expression element, particularly active in the secondary xylem or older stems. In this study, the 1127 bp 5′- upstream region of the 4CL1 coding sequence from Eucalyptus camaldulensis, Euc4CL1, was isolated and characterized. Essential putative cis-elements in the Euc4CL1 promoter included: a TATA-box at −22/−28 position, two CCAAT-boxes at −256/−260 and −277/−281 positions, respectively, an AC-element at −328/−336 and A-boxes at −115/−120 and −990/−995 positions. To investigate the effect of the Euc4CL1 promoter on gene expression, a plant transformation vector, pEuc4CL1p, containing the reporter gene for β-glucuronidase (GUS) under the control of Euc4CL1 promoter was constructed based on the pBI101 backbone and introduced in tobacco plants. Stable expression of the GUS gene in transgenic lines was analysed by a histochemical GUS assay. The results indicated the specific expression of the GUS gene in the stem xylem cells of transgenic tobacco lines was controlled by the Euc4CL1 promoter. The observations suggest the isolated Euc4CL1 promoter is a potential candidate for driving the expression of a foreign gene in plant xylem tissues.