Healing mechanisms of the hydroalcoholic extract and ethyl acetate fraction of green tea (Camellia sinensis (L.) Kuntze) on chronic gastric ulcers

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• 作者：Débora Gasparin Borato…
• 关键词：Camellia sinensis ; Green tea ; Gastric ulcer healing ; Mucin
• 刊名：Naunyn-Schmiedeberg's Archives of Pharmacology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：389
• 期：3
• 页码：259-268
• 全文大小：1,419 KB
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• 作者单位：Débora Gasparin Borato (1)
  Camila Toledo Scoparo (2)
  Daniele Maria-Ferreira (1)
  Luísa Mota da Silva (1)
  Lauro Mera de Souza (2)
  Marcello Iacomini (2)
  Maria Fernanda de Paula Werner (1)
  Cristiane Hatsuko Baggio (1)
  
  1. Department of Pharmacology, Sector of Biological Sciences, Federal University of Parana, PO Box 19031, Curitiba, PR, 81531-980, Brazil
  2. Department of Biochemistry and Molecular Biology, Sector of Biological Sciences, Federal University of Parana, Curitiba, PR, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Pharmacology and Toxicology
  Neurosciences
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1912

文摘

Green tea is an infusion of unfermented leaves of Camellia sinensis (L.) Kuntze (Theaceae), traditionally used for the treatment of obesity, hypercholesterolemia, and gastric complaints. This study evaluated the mechanisms involved in the gastric ulcer healing of the hydroalcoholic extract from green tea (GEt), its ethyl acetate fraction, (GEAc) and epigallocatechin gallate (EGCG) using the model of acetic acid-induced gastric ulcer in rats. The chronic gastric ulcer was induced by application of 80 % acetic acid on serosal mucosa of rats. After 7 days of oral treatment with GEt and GEAc, the ulcer area, mucin content, inflammatory parameters (MPO and NAG), and antioxidant system (GSH and LOOH levels, SOD and GST activities) were evaluated. In vitro, the scavenging activity of GEt and GEAc were also measured. The antisecretory action was studied on the pylorus ligature method in rats. Oral treatment with GEt and GEAc reduced significantly the gastric ulcer area induced by acetic acid. The gastric ulcer healing was accompanied by increasing of mucin content, restoration of GSH levels and SOD activity, and reduction of MPO and LOOH levels. In addition, GEt and GEAc reduced the DPPH free radicals in vitro. Furthermore, the oral treatment of animals with GEt and GEAc did not alter the gastric acid secretion or cause signs of toxicity. Collectively, these results showed that GEt had a pronounced antiulcer effect, possibly through maintenance of mucin content and reduction of inflammation and oxidative stress. In addition, the compounds present in its ethyl acetate fraction could be responsible for the extract activity. Keywords Camellia sinensis Green tea Gastric ulcer healing Mucin