External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells

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• 作者：Xin Yan (12)
  Feng Li (3)
  Igor Dozmorov (4)
  Mark Barton Frank (4)
  Ming Dao (5)
  Michael Centola (4)
  Wei Cao (3)
  Dan Hu (6) dan_hu@rics.bwh.harvard.edu
• 关键词：Small ; cell lung cancer &#8211 ; Gene expression &#8211 ; Cell death &#8211 ; Anti ; cancer effect
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：363
• 期：1-2
• 页码：245-255
• 全文大小：689.3 KB
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• 作者单位：1. The Institute of Chongqing Traditional Chinese Medicine, Chongqing, China2. New Medical Science Research Institute, New York, NY 10107, USA3. University of Oklahoma Health Sciences Center, Oklahoma, OK 73104, USA4. Microarray Research Facility, Oklahoma Medical Research Foundation, Oklahoma, OK 73104, USA5. Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA6. Center for Neurologic Diseases, Brigham and Women鈥檚 Hospital, Harvard Medical School, HIM, Room 730, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.