IL-6 originated from breast cancer tissue-derived mesenchymal stromal cells may contribute to carcinogenesis

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• 作者：?zlem Sa?lam ; Zehra Seda ünal ; Cansu Suba?? ; Engin Ulukaya ; Erdal Kara?z
• 关键词：IL ; 6 ; Tumor microenvironment ; Mesenchymal stromal cells ; Breast cancer
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：36
• 期：7
• 页码：5667-5677
• 全文大小：2,045 KB
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  24.Khamis ZI, Sahab ZJ, Amy Sang Q. Active roles
• 作者单位：?zlem Sa?lam (1)
  Zehra Seda ünal (1)
  Cansu Suba?? (1)
  Engin Ulukaya (2)
  Erdal Kara?z (3)
  
  1. Center for Stem Cell and Gene Therapies Research and Practice, Department of Stem Cell, Kocaeli University, Kocaeli, Turkey
  2. Department of Medical Biochemistry, Uluda? University, Bursa, Turkey
  3. Liv Hospital, Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell), ?stanbul, Turkey
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

Tumor microenvironment is an important factor, which sustains and promotes the tumors by inflammatory signals. Interleukin-6 (IL-6) is known as a multifunctional cytokine, which is a major activator of the signaling pathway of Janus kinases (JAKs)/signal transducer and activator of transcription 3 (STAT3). In this study, we aimed to investigate the effect of IL-6 in the tumor microenvironment on carcinogenesis. For this purpose, healthy breast tissue-derived stromal cells (HBT-SCs) and malign breast tissue-derived stromal cells (MBT-SCs) were co-cultured with MCF-7 (human breast adenocarcinoma cell line) cells using semipermeable membranes. The cell proliferation was monitored with water-soluble tetrazolium (WST) and carboxyfluorescein succinimidyl ester (CFSE) assays. Protein levels were measured by enzyme-linked immunosorbent assay (ELISA) and Western blot hybridization, while gene expressions were measured by real-time PCR. The results demonstrated that IL-6 protein levels increased significantly in the supernatants of MBT-SCs when they were co-cultured with MCF-7 cells. In accordance with this, the expression of IL-6 was significantly higher in MBT-SCs. Additionally, the expression of STAT3 in MCF-7 cells increased slightly when they were co-cultured with MBT-SCs. Considering together, there is an important interaction between tumor microenvironment and tumor cells mediated by IL-6 signaling. Thereby, the targeting on IL-6 signaling in the treatment of cancer might effectively prevent the tumor progression.