Potential of inherent RGD containing silk fibroin–poly (Є-caprolactone) nanofibrous matrix for bone tissue engineering

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• 作者：Promita Bhattacharjee ; Banani Kundu ; Deboki Naskar ; Hae-Won Kim…
• 关键词：Non ; mulberry silk fibroin ; Poly (Є ; caprolactone) ; Nanofibers ; Bone tissue engineering
• 刊名：Cell and Tissue Research
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：363
• 期：2
• 页码：525-540
• 全文大小：13,951 KB
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• 作者单位：Promita Bhattacharjee (1)
  Banani Kundu (2) (3)
  Deboki Naskar (2)
  Hae-Won Kim (3)
  Debasis Bhattacharya (1)
  T. K. Maiti (2)
  S. C. Kundu (2)
  
  1. Materials Science Centre, Indian Institute of Technology Kharagpur, West Bengal, 721302, India
  2. Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, 721302, India
  3. Institute of Tissue Regeneration Engineering (ITREN) & Department of Nanobiomedical Science BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, South Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

The current study deals with the fabrication and characterization of blended nanofibrous scaffolds of tropical tasar silk fibroin of Antheraea mylitta and poly (Є-caprolactone) to act as an ideal scaffold for bone regeneration. The use of poly (Є-caprolactone) in osteogenesis is well-recognized. At the same time, the osteoconductive nature of the non-mulberry tasar fibroin is also established due to its internal integrin binding peptide RGD (Arg-Gly-Asp) sequences, which enhance cellular interaction and proliferation. Considering that the materials have the required and favorable properties, the blends are formed using an equal volume ratio of fibroin (2 and 4 wt%) and poly (Є-caprolactone) solution (10 wt%) to fabricate nanofibers. The nanofibers possess an average diameter of 152 ± 18 nm (2 % fibroin/PCL) and 175 ± 15 nm (4 % fibroin/PCL). The results of Fourier transform infrared spectroscopy substantiates the preservation of the secondary structure of the fibroin in the blends indicating the structural stability of the neo-matrix. With an increase in the fibroin percentage, the hydrophobicity and thermal stability of the matrices as measured from melting temperature T_m (using DSC) decrease, while the mechanical strength is improved. The blended nanofibrous scaffolds are biodegradable, and support the viability and proliferation of human osteoblast-like cells as observed through scanning electron and confocal microscopes. Alkaline phosphatase assay indicates the cell proliferation and the generation of the neo-bone matrix. Taken together, these findings illustrate that the silk–poly (Є-caprolactone) blended nanofibrous scaffolds have an excellent prospect as scaffolding material in bone tissue engineering. Keywords Non-mulberry silk fibroin Poly (Є-caprolactone) Nanofibers Bone tissue engineering