A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

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• 作者：Abdoulkarim Ibrahim Issa ; Jean-Noël Duprez…
• 关键词：Azawak zebu ; Mastitis ; Staphylococcus aureus ; Pulsed field gel electrophoresis ; Virulotyping ; PCR
• 刊名：Tropical Animal Health and Production
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：48
• 期：2
• 页码：321-329
• 全文大小：385 KB
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• 作者单位：Abdoulkarim Ibrahim Issa (1) (2)
  Jean-Noël Duprez (1)
  Rianatou Bada-Alambedji (3)
  Mamane Djika (4)
  Jacques Georges Mainil (1)
  Marjorie Bardiau (1) (5)
  
  1. Department of Infectious Diseases (Bacteriology), Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animal Health (FARAH), University of Liège, Campus du Sart Tilman (B43a), Quartier Vallée II, Avenue de Cureghem 6, 4000, Liège, Belgium
  2. Department of Diagnosis, Epidemiological Investigations and Applied Research, Laboratoire Central d’Elevage (LABOCEL), BP 485, Niamey, Niger
  3. Department of Immunology and Infectious Pathology (Microbiology), Interstate School of Veterinary Science and Medicine, BP 5077, Dakar, Senegal
  4. National School of Public Health, BP 290, Niamey, Niger
  5. Present address: Environment & Public Health Research Group, School of Environment & Technology, University of Brighton, Cockcroft Building, Lewes Road, Brighton, BN2 4GJ, UK
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Veterinary medicine
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-7438

文摘

Staphylococcus (S.) aureus is one of the most important pathogens causing bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental station of Toukounous (Niger) by (i) comparing their pulsed field gel electrophoresis (PFGE) fingerprints, (ii) identifying their virulotypes by PCR amplification and (iii) assessing the production of capsule and the formation of biofilm. The 88 S. aureus isolates belonged to 14 different pulsotypes, 3 of them being predominant: A (30 %), D (27 %), B (15 %). A and B pulsotypes had the highest profile similarity coefficient (94 %), while others had similarity coefficients under 60 %. Seventy-five S. aureus isolates were further studied for their virulotypes, capsular antigens and biofilm production. Most surface factor-, leukocidin- and haemolysin-, but not the enterotoxin-encoding genes were detected in the majority (>75 %) of the isolates and were evenly distributed between the A, B and D pulsotype isolates. The majority of the 72 S. aureus positive with the cap5H or cap8H PCR produced the CP5 (82 %) or the CP8 (88 %) capsular antigen, respectively. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates, moderate for 31 isolates but weak for 18 isolates, implying that the icaA gene may not be expressed in vitro by one third of the positive isolates. Similar to other studies, those results confirm that a restricted number of S. aureus clones circulate within the three herds at Toukounous and that their specific virulence-associated properties must still be further studied. Keywords Azawak zebu Mastitis Staphylococcus aureus Pulsed field gel electrophoresis Virulotyping PCR