Cyclic voltammetric acetylcholinesterase biosensor for the detection of captan in apple samples with the aid of chemometrics

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• 作者：Noel Nesakumar ; Swaminathan Sethuraman…
• 关键词：Acetylcholinesterase biosensor ; ZnO nanorods ; Captan ; Acetylthiocholine chloride ; Cyclic voltammetry
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：407
• 期：16
• 页码：4863-4868
• 全文大小：754 KB
• 参考文献：1.Smith FD, MacHardy WE (1984) Phytopathol 74:894-99View Article
  2.Singh RP, Kim YJ, Oh BK, Choi JW (2009) Electrochem Commun 11:181-85View Article
  3.Rawn DF, Quade SC, Shields JB, Conca G, Sun WF, Smith M, Fouquet A, Belanger A (2007) Food Addit Contam 24:149-55View Article
  4.Chauhan N, Narang J, Pundir CS (2011) Int J Biol Macromol 49:923-29View Article
  
• 作者单位：Noel Nesakumar (1) (2) (4)
  Swaminathan Sethuraman (2)
  Uma Maheswari Krishnan (2) (3)
  John Bosco Balaguru Rayappan (1) (2) (4)
  
  1. Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  2. Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  4. School of Electrical & Electronics Engineering, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  3. School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The presence of captan residues in apples shows high toxicity, which often causes eye and skin irritation, dermatitis, conjunctivitis, and vomiting in humans. In this context, an electrochemical biosensor based on acetylcholinesterase (AChE) immobilized on a ZnO nanorod interface has been proposed. In this work, Hill, dose-response, and first-, second-, and third-order polynomial regression models were successfully applied and the prediction ability of these models was tested with the use of current density obtained from the cyclic voltammograms of appropriate captan solutions. The Pt/ZnO/AChE bioelectrode showed a high sensitivity of 0.538?μA?cm??μM? in the linear range from 0.05 to 25.0?μM with a limit of detection of 107?nM. The recovery results were observed between 98.4 and 102.4?% from the apple sample. This work provides a new promising tool for the detection of captan in apple samples.