Carbamazepine-mediated pro-oxidant effects on the unicellular marine algal species Dunaliella tertiolecta and the hemocytes of mussel Mytilus galloprovincialis
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- 作者：Pinelopi Tsiaka ; Vasiliki Tsarpali ; Ioanna Ntaikou ; Maria N. Kostopoulou…
- 关键词：Carbamazepine ; Dunaliella ; Hemocytes ; Lipid peroxidation ; Oxidative stress ; related effects ; Toxicity
- 刊名：Ecotoxicology
- 出版年：2013
- 出版时间：October 2013
- 年：2013
- 卷：22
- 期：8
- 页码：1208-1220
- 全文大小：500KB
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Vasiliki Tsarpali (1)
Ioanna Ntaikou (2)
Maria N. Kostopoulou (3)
Gerasimos Lyberatos (4)
Stefanos Dailianis (1) (5)
1. Section of Animal Biology, Department of Biology, School of Natural Sciences, University of Patras, 26500, Patras, Greece
2. Institute of Chemical Engineering Sciences, Foundation of Research and Technology Hellas (ICEHT/FORTH), 10 Stadiou st., Platani, 26504, Patras, Greece
3. Department of Marine Sciences, School of the Environment, University of the Aegean, University Hill, 81100, Mytilene, Greece
4. School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 15780, Athens, Greece
5. Section of Animal Biology, Department of Biology, Faculty of Sciences, University of Patras, 26500, Patras, Greece - ISSN：1573-3017
文摘
This study investigates the pro-oxidant behavior of the antiepileptic drug carbamazepine (CBZ) on the marine algal species Dunaliella tertiolecta and the immune defense-related hemocytes of mussel Mytilus galloprovincialis. A phytotoxicity test, performed in a first step, showed a significant inhibition of the growth rate and the chlorophyll alpha (Chl-α) content in algae after exposure for 24?h to different concentrations of CBZ (1-00?mg?L?). On the other hand, the increased levels of lipid peroxidation products, such as MDA, measured in 24?h CBZ-treated cells were attenuated with time (48-6?h), followed by a significant recovery of both the algal growth rate and the Chl-α content in all cases. The latter could be related to the concomitant enhancement of total carotenoids in CBZ-treated algae with time, which in turn could protect algal growth and survival against CBZ-induced oxidative stress. On the other hand, the increased levels of cell death, superoxide anions (·O2 ?/sup>), nitric oxides (NO, in terms of nitrites, NO2 ?/sup>) and MDA content observed in mussel hemocytes exposed to environmentally relevant (0.01-?μg?L?) and/or higher (10 and 100?μg?L?) concentrations of the drug, clearly indicate the ability of CBZ to induce oxidative effects on cells of non-target species, such as mussels, affecting thus their overall health status. The significant relationships occurred among the tested biological parameters in both bioassays, further reinforce CBZ-mediated pro-oxidant effects on species, widely used in ecotoxicological and toxicological studies and provide a more comprehensive view on its environmental fate and ecotoxicological risk evaluation.