Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry

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• 作者：Marcos Tascon ; Fernando Benavente…
• 关键词：Capillary electrophoresis ; Mass spectrometry ; MS–MS ; Undaria pinnatifida ; Harmala alkaloids ; Validation
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：407
• 期：13
• 页码：3637-3645
• 全文大小：844 KB
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• 作者单位：Marcos Tascon (1)
  Fernando Benavente (3)
  Victoria M. Sanz-Nebot (3)
  Leonardo G. Gagliardi (1) (2)
  
  1. Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA), Universidad Nacional de La Plata, Calle 47 y 115, 1900, La Plata, Buenos Aires, Argentina
  3. Departamento de Química Analítica, Nutrition and Food Safety Research Institute (INSA-UB), Universidad de Barcelona, Av. Diagonal 645, 08028, Barcelona, Espa?a
  2. Centro de Investigación y Desarrollo en Tecnología de Pinturas (CIDEPINT-CIC-CONICET), Av. 52 y 120, 1900, La Plata, Buenos Aires, Argentina
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (Undaria pinnatifida), an edible marine invasive algae. HAlks are based on a characteristic β-carboline structure with at least one amino ionizable group. In this work, we report the successful separation of a mixture of six HAlks (harmine, harmaline, harmol, harmalol, harmane, and norharmane) by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) in less than 8?min. Optimum separation in fused-silica capillaries and detection sensitivity in positive-ion mode were achieved using a background electrolyte (BGE) with 25?mmol?L? ammonium acetate (pH?7.8) and 10?% (v/v) methanol, and a sheath liquid with 60:40 (v/v) isopropanol–water and 0.05?% (v/v) formic acid. The separation method was validated in terms of linearity, limits of detection and quantification, repeatability, and reproducibility. Later, a sample pretreatment was carefully optimized to determine HAlks in commercial wakame samples with excellent recovery and repeatability. For the complex wakame extracts, the MS–MS fragmentation patterns of the different HAlks were useful to ensure a reliable identification. The complete procedure was validated using the standard-addition calibration method, determining matrix effects on the studied compounds. Harmalol, harmine, and harmaline were naturally present in the samples and were quantified at very low concentrations, ranging from 7 to 24?μg?kg? dry algae.