Regulating Pyruvate Carboxylase in the Living Culture of Aspergillus Terreus Nrrl 1960 by l-Aspartate for Enhanced Itaconic Acid Production

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• 作者：Pajareeya Songserm ; Sitanan Thitiprasert…
• 关键词：Itaconic acid ; l ; aspartate ; Pyruvate carboxylase ; Cis ; aconitate decarboxylase ; 6 ; phosphofructo ; 1 ; kinase ; Aspergillus terreus ; Allosteric inhibition ; Fermentation
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：177
• 期：3
• 页码：595-609
• 全文大小：905 KB
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• 作者单位：Pajareeya Songserm (1)
  Sitanan Thitiprasert (2)
  Vasana Tolieng (2)
  Jiraporn Piluk (2)
  Somboon Tanasupawat (3)
  Sutthichai Assabumrungrat (4)
  Shang-Tian Yang (5)
  Aphichart Karnchanatat (2)
  Nuttha Thongchul (2)
  
  1. Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand
  2. Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand
  3. Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand
  4. Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand
  5. William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

Aspergillus terreus was reported as the promising fungal strain for itaconic acid; however, the commercial production suffers from the low yield. Low production yield was claimed as the result of completing the tricarboxylic acid (TCA) cycle towards biomass synthesis while under limiting phosphate and nitrogen; TCA cycle was somewhat shunted and consequently, the metabolite fluxes move towards itaconic acid production route. By regulating enzymes in TCA cycle, it is believed that itaconic acid production can be improved. One of the key responsible enzymes involved in itaconic acid production was triggered in this study. Pyruvate carboxylase was allosterically inhibited by l-aspartate. The presence of 10 mM-span class="EmphasisTypeSmallCaps ">l-aspartate in the production medium directly repressed PC expression in the living A. terreus while the limited malate flux regulated the malate/citrate antiporters resulting in the increasing cis-aconitate decarboxylase activity to simultaneously convert cis-aconitate, citrate isomer, into itaconic acid. The transport of cis-aconitate via the antiporters induced citrate synthase and 6-phosphofructo-1-kinase activities in response to balance the fluxes of TCA intermediates. Successively, itaconic acid production yield and final concentration could be improved by 8.33 and 60.32 %, respectively, compared to those obtained from the control fermentation with the shortened lag time to produce itaconic acid during the production phase. Keywords Itaconic acid l-aspartate Pyruvate carboxylase Cis-aconitate decarboxylase 6-phosphofructo-1-kinase Aspergillus terreus Allosteric inhibition Fermentation