Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment

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• 作者：Carlos Araújo ; Estefanía Mu?oz-Atienza…
• 关键词：Rainbow trout (Oncorhynchus mykiss) ; Probiotics ; Lactococcus lactis ; Safety assessment ; Genetic relatedness ; ERIC ; PCR ; Nisin Z
• 刊名：European Food Research and Technology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：241
• 期：5
• 页码：647-662
• 全文大小：883 KB
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  25.Araújo C, Mu?oz-Atienza E, Nahuelquín Y, Poeta P, Igrejas G, Hernández PE, Herranz C, Cintas LM (2014) Inhibition of fish pathogens by the microbiota from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment. Anaerobe 32:7-4CrossRef
  26.Mu?oz-Atienza E, Gómez-Sala B, Araújo C, Campanero C, del Campo R, Hernández PE, Herranz C, Cintas LM (2013) Antimicrobial activity, antibiotic susceptibility and virulence factors of lactic acid bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC Micro
• 作者单位：Carlos Araújo (1) (5)
  Estefanía Mu?oz-Atienza (1)
  Martha Ramírez (1)
  Patrícia Poeta (2) (3)
  Gilberto Igrejas (4) (5)
  Pablo E. Hernández (1)
  Carmen Herranz (1)
  Luis M. Cintas (1)
  
  1. Grupo de Seguridad y Calidad de los Alimentos por Bacterias Lácticas, Bacteriocinas y Probióticos (Grupo SEGABALBP), Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain
  5. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  2. Centre for Animal Science and Veterinary, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  3. Veterinary Science Department, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  4. Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Analytical Chemistry
  Biotechnology
  Agriculture
  Forestry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-2385

文摘

This work reports the safety assessment, genetic relatedness and extracellular antimicrobial (bacteriocin) activity of 75 potential probiotic Lactococcus lactis strains isolated from aquacultured rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment, and the biochemical and genetic characterization of the bacteriocin most active against the rainbow trout pathogen Lactococcus garvieae. Seventeen (22 %) strains produced, at least, one biogenic amine, but none of them amplified the corresponding genes. Thirty (40 %) strains showed antibiotic resistance to, at least, one antibiotic, and the only antibiotic resistance genes found were tet(K), tet(L), tet(O) and tet(T). None of the strains produced hemolysin or gelatinase, degraded gastric mucin or deconjugated bile salts. ERIC-PCR fingerprinting allowed clustering the lactococci in three well-defined and highly divergent groups (31.0 % similarity). Nine (12 %) L. lactis strains were identified as bacteriocin producers. The bacteriocin produced by L. lactis subsp. cremoris WA2-67 was purified, and mass spectrometry and DNA sequencing revealed its identity to nisin Z (NisZ). Altogether, the results allowed the identification of 34 (45.3 %) putatively safe lactococci, including three bacteriocinogenic strains. This is the first description of tet(K), tet(O) and tet(T) in L. lactis. Moreover, ERIC-PCR fingerprinting has been shown for the first time as a valuable tool for genetic profiling of L. lactis strains from aquatic origin. A NisZ-producing L. lactis strain has been isolated for the first time from an aquatic environment, and this strain may be considered as a potential probiotic for rainbow trout farming for improving the quality and safety of fish and fish products. Keywords Rainbow trout (Oncorhynchus mykiss) Probiotics Lactococcus lactis Safety assessment Genetic relatedness ERIC-PCR Nisin Z