Transcriptomics of differential vector competence: West Nile virus infection in two populations of Culex pipiens quinquefasciatus linked to ovary development

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• 作者：Dongyoung Shin (17)
  Ayse Civana (17)
  Carolina Acevedo (17)
  Chelsea T Smartt (17)
  
  17. Florida Medical Entomology Laboratory ; University of Florida ; 200 9th St. S.E. ; Vero Beach ; FL ; 32962 ; USA
  
• 关键词：Culex pipiens quinquefasciatus ; West Nile virus (WNV) ; High throughput RNA sequencing ; Vector competence
• 刊名：BMC Genomics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：15
• 期：1
• 全文大小：625 KB
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• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background Understanding mechanisms that contribute to viral dissemination in mosquito vectors will contribute to our ability to interfere with the transmission of viral pathogens that impact public health. The expression of genes in two Culex pipiens quinquefasciatus populations from Florida with known differences in vector competence to West Nile virus (WNV) were compared using high throughput sequencing. Results A total of 15,176 transcripts were combined for comparison of expression differences between the two populations and 118 transcripts were differentially expressed (p RNAseq data analysis, ovary development was compared across the populations and following WNV infection. There were significant differences among the compared groups. Conclusions This study suggests that ovary development is correlated to vector competence in two Culex populations in Florida. Both populations control energy allocations to reproduction as a response to WNV. This result provides novel insight into the defense mechanism used by Culex spp. mosquitoes against WNV.