Amebicidal activity of the essential oils of Lippia spp. (Verbenaceae) against Acanthamoeba polyphaga trophozoites
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- 作者：Israel Gomes de Amorim Santos ; Ricardo Scher…
- 关键词：Acanthamoeba polyphaga ; Lippia spp. ; Carvacrol ; Carvone ; Rotundifolone ; Amebicidal activity
- 刊名：Parasitology Research
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：115
- 期：2
- 页码：535-540
- 全文大小：409 KB
- 参考文献：Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry. Allured Pub Corp, USA
Botelho MA, Nogueira NA, Bastos GM, Fonseca SG, Lemos TL, Matos FJ, Montenegro D, Heukelbach J, Brito GA, Rao VS (2007) Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Braz J Med Biol Res 40(3):349–356CrossRef PubMed
Clardy J, Walsh C (2004) Lessons from natural molecules. Nature 432(7019):829–837CrossRef PubMed
Clarke DW, Niederkorn JY (2006) The pathophysiology of Acanthamoeba keratitis. Trends Parasitol 22(4):175–180CrossRef PubMed
Dart JK, Saw VPJ, Kilvington S (2009) Acanthamoeba keratitis: diagnosis and treatment update 2009. Am J Ophthalmol 148(4):487–499CrossRef PubMed
Degerli S, Tepe B, Celiksoz A, Berk S, Malatyali E (2012) In vitro amoebicidal activity or Origanum syriacum and Origanum laevigatum on Acanthamoeba castellani cysts and trophozoites. Exp Parasitol 131(1):20–24CrossRef PubMed
Escobar P, Milena Leal S, Herrera LV, Martinez JR, Stashenko E (2010) Chemical composition and antiprotozoal activities of Colombian Lippia spp essential oils and their major components. Mem Inst Oswaldo Cruz 105(2):184–190CrossRef PubMed
Farias-Junior PA, Rios MC, Moura TA, Almeida RP, Alves PB, Blank AF, Fernandes RP, Scher R (2012) Leishmanicidal activity of carvacrol-rich essential oil from Lippia sidoides Cham. Biol Res 45(4):399–402CrossRef PubMed
Khan NA, Siddiqui R (2009) Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction protein. Int J Parasitol 39(14):1611–1616CrossRef PubMed
Lima GP, Souza TM, Paula Freire G, Farias DF, AP C, Ricardo NM, de Morais SM, Carvalho AF (2013) Further insecticidal activities of essential oils from Lippia sidoides and Croton species against Aedes aegypti L. Parasitol Res 112(5):1953–1958CrossRef PubMed
Lorenzo-Morales J, Khan NA, Walochnik J (2015) An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite 22:10PubMedCentral CrossRef PubMed
Malatyali E, Tepe B, Degerli S, Berk S, Akpulat HA (2012) In vitro amoebicidal activity of four Peucedanum species on Acanthamoeba castellanii cysts and trophozoites. Parasitol Res 110(1):167–174CrossRef PubMed
Melo JO, Bitencourt TA, Fachin AL, Cruz EM, Jesus HC, Alves PB, Fátima Arrigoni-Blank M, Castro Franca S, Beleboni RO, Fernandes RP, Blank AF, Scher R (2013) Antidermatophytic and antileishmanial activities of essential oils from Lippia gracilis Schauer genotypes. Acta Trop 128(1):110–115CrossRef PubMed
Menezes LR, Santos NN, Dos Santos JA, Meira CS, Guimaraes ET, Soares MB, Nepel A, Barisone A, Costa EV (2014) A new source of (R)-limonene and rotundifolone from leaves of Lippia pedunculosa (Verbenaceae) and their trypanocidal properties. Nat Prod Commun 9(6):737–739
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1-2):55–63CrossRef PubMed
Ocazionez RE, Meneses R, Torres FA, Stashenko E (2010) Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro. Mem Inst Oswaldo Cruz 105(3):304–309CrossRef PubMed
Petry F, Torzewski M, Bohl J, Wilhelm-Schwenkmezger T, Scheid P, Walochnik J, Michel R, Zöller L, Werhahn KJ, Bhakdi S, Lackner KJ (2006) Early diagnosis of Acanthamoeba infection during routine cytological examination of cerebrospinal fluid. J Clin Microbiol 44(5):1903–1904PubMedCentral CrossRef PubMed
Polat ZA, Vural A, Ozan F, Tepe B, Özcelik S, Cetin A (2008) In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. J Ocul Pharmacol Ther 24(1):8–14CrossRef PubMed
Rodrigues FAR, Bomfim IS, Cavalcanti BC, Pessoa C, Gonçalves RSB, Wardell JL, Wadell SMSV, Souza MVN (2014) Mefloquine–oxazolidine derivatives: a new class of anticancer agents. Chem Biol Drug Des 83:126–131CrossRef PubMed
Sauter IP, Santos JC, Apel MA, Cibulski SP, Roehe PM, von Poser GL, Rott MB (2011) Amoebicidal activity and chemical composition of Pterocaulon polystachyum (Asteraceae) essential oil. Parasitol Res 109(5):1367–1371CrossRef PubMed
Sauter IP, Rossa GE, Lucas AM, Cibulski SP, Roehe PM, Silva LAA, Rott MB, Vargas RMF, Cassel E, von Poser GL (2012) Chemical composition and amoebicidal activity of Piper hispidinervum (Piperaceae) essential oil. Ind Crop Prod 40:292–295CrossRef
Scheid P, Zöller L, Pressmar S, Richard G, Michel R (2008) An extraordinary endocytobiont in Acanthamoeba sp. isolated from a patient with keratitis. Parasitol Res 102(5):945–950CrossRef PubMed
Sifaoui I, López-Arencibia A, Martín-Navarro CM, Chammem N, Mejri M, Lorenzo-Morales J, Abderabba M, Piñero JE (2013) Activity assessment of Tunisian olive leaf extracts against the trophozoite stage of Acanthamoeba. Parasitol Res 113(2):755–759PubMed
Vandendool H, Kratz PD (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J Chromatogr 11:463–471CrossRef PubMed
Visvesvara GS (2010) Amebic meningoencephalitides and keratitis: challenges in diagnosis and treatment. Curr Opin Infect Dis 23(6):590–594CrossRef PubMed
Vunda SL, Sauter IP, Cibulski SP, Roehe PM, Bordignon SA, Rott MB, Apel MA, von Pose GL (2012) Chemical composition and amoebicidal activity of Croton pallidulus, Croton ericoides, and Croton isabelli (Euphorbiaceae) essential oils. Parasitol Res 111(3):961–966CrossRef PubMed - 作者单位：Israel Gomes de Amorim Santos (1)
Ricardo Scher (2)
Marilise Brittes Rott (3)
Leociley Rocha Menezes (4)
Emmanoel Vilaça Costa (5)
Sócrates Cabral de Holanda Cavalcanti (6)
Arie Fitzgerald Blank (7)
Jaciana dos Santos Aguiar (8)
Teresinha Gonçalves da Silva (8)
Silvio Santana Dolabella (1)
1. Departamento de Morfologia, Laboratório de Entomologia e Parasitologia Tropical, Universidade Federal de Sergipe, Av. Marechal Rondon, s/n, Roza Elze, CEP 49100-000, São Cristóvão, Sergipe, Brazil
2. Departamento de Morfologia, Laboratório de Biologia Molecular de Bioagentes Patogênicos, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil
3. Instituto de Ciências Básicas da Saúde, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
4. Departamento de Química, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil
5. Departamento de Química, Universidade Federal do Amazonas, Manaus, Amazonas, Brazil
6. Departamento de Farmácia, Laboratório de Química Farmacêutica, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil
7. Departamento de Agronomia, Laboratório de Fitotecnia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil
8. Departamento de Antibióticos, Laboratório de Bioensaios para Pesquisa de Fármacos, Universidade Federal de Pernambuco, Recife, Brazil - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biomedicine
Medical Microbiology
Microbiology
Immunology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-1955
文摘
Amoebic keratitis and granulomatous amoebic encephalitis are caused by some strains of free-living amoebae of the genus Acanthamoeba. In the case of keratitis, one of the greatest problems is the disease recurrence due to the resistance of parasites, especially the cystic forms, to the drugs that are currently used. Some essential oils of plants have been used as potential active agents against this protist. Thus, the aim of this study was to determine the amebicidal activity of essential oils from plants of the genus Lippia against Acanthamoeba polyphaga trophozoites. To that end, 8 × 104 trophozoites were exposed for 24 h to increasing concentrations of essential oils from Lippia sidoides, Lippia gracilis, Lippia alba, and Lippia pedunculosa and to their major compounds rotundifolone, carvone, and carvacrol. Nearly all concentrations of oils and compounds showed amebicidal activity. The IC50 values for L. sidoides, L. gracilis L. alba, and L. pedunculosa were found to be 18.19, 10.08, 31.79, and 71.47 μg/mL, respectively. Rotundifolone, carvacrol, and carvone were determined as the major compounds showing IC50 of 18.98, 24.74, and 43.62 μg/mL, respectively. With the exception of oil from L. alba, the other oils evaluated showed low cytotoxicity in the NCI-H292 cell line. Given these results, the oils investigated here are promising sources of compounds for the development of complementary therapy against amoebic keratitis and granulomatous amoebic encephalitis and can also be incorporated into cleaning solutions to increase their amebicidal efficiency. Keywords Acanthamoeba polyphaga Lippia spp. Carvacrol Carvone Rotundifolone Amebicidal activity