Effect of Amniotic Membrane Proteins in Human Cancer Cell Lines: An Exploratory Study

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• 作者：Ana Catarina Mamede (1) (2) (3) (4)
  M. Laranjo (1) (3) (4)
  M. J. Carvalho (1) (3) (4) (5)
  A. M. Abrantes (1) (3) (4)
  A. S. Pires (1) (4) (6)
  A. F. Brito (1) (3) (4)
  P. Moura (5)
  C. J. Maia (2)
  M. F. Botelho (1) (3) (4)
  
• 关键词：Amniotic membrane ; Cancer cell lines ; Metabolic activity ; MTT assay
• 刊名：Journal of Membrane Biology
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：247
• 期：4
• 页码：357-360
• 全文大小：172 KB
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  3. Jiao H, Guan F, Yang B, Li J, Song L, Hu X, Du Y (2012) Human amniotic membrane derived-mesenchymal stem cells induce C6 glioma apoptosis in vivo through the Bcl-2/caspase pathways. Mol Biol Rep 39(1):467-73. doi:10.1007/s11033-011-0760-z CrossRef
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  5. Kang N-H, Hwang K-A, Kim S, Kim Y-B, Hyun S-H, Jeung E-B, Choi K-C (2012b) Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells. Cancer Gene Ther 19(8):517-22. doi:10.1038/cgt.2012.30 CrossRef
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  7. Mamede AC, Carvalho MJ, Abrantes AM, Laranjo M, Maia CJ, Botelho MF (2012a) Amniotic membrane: from structure and functions to clinical applications. Cell Tissue Res 349(2):447-58. doi:10.1007/s00441-012-1424-6 CrossRef
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  10. Miki T, Marongiu F, Dorko K, Ellis EC, Strom SC (2010) Isolation of amniotic epithelial stem cells. Curr Protoc Stem Cell Biol Suppl 1E.3.1.-E.3.10. doi:10.1002/9780470151808.sc01e03s12
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• 作者单位：Ana Catarina Mamede (1) (2) (3) (4)
  M. Laranjo (1) (3) (4)
  M. J. Carvalho (1) (3) (4) (5)
  A. M. Abrantes (1) (3) (4)
  A. S. Pires (1) (4) (6)
  A. F. Brito (1) (3) (4)
  P. Moura (5)
  C. J. Maia (2)
  M. F. Botelho (1) (3) (4)
  
  1. Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal
  2. CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilh?, Portugal
  3. Centre of Investigation on Environment, Genetics and Oncobiology - CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
  4. Institute for Biomedical Imaging and Life Sciences - IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
  5. Obstetrics Service, Coimbra Hospital and University Centre, Coimbra, Portugal
  6. Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal
  
• ISSN：1432-1424

文摘

Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50?% in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50?% inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable.