Interaction between Saccharomyces cerevisiae mitochondrial DNA-binding protein Abf2p and Cce1p resolvase

详细信息    查看全文

• 作者：E. O. Samoilova ; I. A. Krasheninnikov ; S. A. Levitskii
• 关键词：Key wordsmitochondrion ; homologous recombination ; Cce1p ; Abf2p
• 刊名：Biochemistry (Moscow)
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：81
• 期：10
• 页码：1111-1117
• 全文大小：696 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Microbiology
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3040
• 卷排序：81

文摘

Mitochondrial DNA is susceptible to the action of reactive oxygen species generated by the reactions of oxidative phosphorylation. Homologous recombination is one of the mechanisms providing integrity of the mitochondrial genome. Some proteins that take part in this process in budding yeast mitochondria have been identified. These include Abf2p, the major protein of the mt-nucleoid that specifically binds cruciform DNA, and Cce1p – Holliday junction resolvase. Here we show that Abf2p does not significantly affect either binding of Cce1p to branched DNA or rate and specificity of Holliday junction resolution. These data suggest the existence of an alternative homologous recombination pathway in yeast mitochondria.