Possible involvement of miRNAs in tropism of Parvovirus B19

详细信息    查看全文

• 作者：Azadeh Anbarlou ; Mahshid AkhavanRahnama ; Amir Atashi…
• 关键词：Parvovirus B19 ; Erythroid Progenitors ; microRNAs ; MCF7 ; HEK ; 293
• 刊名：Molecular Biology Reports
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：43
• 期：3
• 页码：175-181
• 全文大小：700 KB
• 参考文献：1.Gallinella G (2013) Parvovirus B19 achievements and challenges. ISRN Virol 2013:1–33CrossRef
  2.Wong S, Zhi N, Filippone C, Keyvanfar K, Kajigaya S, Brown KE, Young NS (2008) Ex vivo-generated CD36 + erythroid progenitors are highly permissive to human parvovirus B19 replication. J Virol 82(5):2470–2476PubMedCentral CrossRef PubMed
  3.Bonvicini F, Filippone C, Delbarba S, Manaresi E, Zerbini M, Musiani M, Gallinella G (2006) Parvovirus B19 genome as a single, two-state replicative and transcriptional unit. Virology 347:447–454CrossRef PubMed
  4.Berillo O, Khailenko V, Ivashchenko A, Perlmuter-Shoshany L, Bolshoy A (2012) miRNA and tropism of human parvovirus B19. Comput Biol Chem 40:1–6CrossRef PubMed
  5.Guan W, Cheng F, Yoto Y, Kleiboeker S, Wong S, Zhi N, Pintel DJ, Qiu J (2008) Block to the production of full-length B19 virus transcripts by internal polyadenylation is overcome by replication of the viral genome. J Virol 82(20):9951–9963PubMedCentral CrossRef PubMed
  6.Winter K, von Kietzell K, Heilbronn R, Pozzuto T, Fechner H, Weger S (2012) Roles of E4orf6 and VA I RNA in adenovirus-mediated stimulation of human parvovirus B19 DNA replication and structural gene expression. J Virol 86(9):5099–5109PubMedCentral CrossRef PubMed
  7.Tewary SK, Zhao H, Deng X, Qiu J, Tang L (2014) The human parvovirus B19 non-structural protein 1 N-terminal domain specifically binds to the origin of replication in the viral DNA. Virology 449:297–303PubMedCentral CrossRef PubMed
  8.Pallier C, Greco A, Le Junter J, Saib A, Vassias I, Morinet F (1997) The 3′untranslated region of the B19 parvovirus capsid protein mRNAs inhibits its own mRNA translation in nonpermissive cells. J Virol 71(12):9482–9489PubMedCentral PubMed
  9.Bonvicini F, Mirasoli M, Manaresi E, Bua G, Calabria D, Roda A, Gallinella G (2013) Single-cell chemiluminescence imaging of parvovirus B19 life cycle. Virus Res 178:517–521CrossRef PubMed
  10.Huang Y, Shen XJ, Zou Q, Wang SP, Tang SM, Zhang GZ (2011) Biological functions of microRNAs: a review. J Physiol Biochem 67(1):129–139CrossRef PubMed
  11.Siomi H, Siomi MC (2010) Posttranscriptional regulation of microRNA biogenesis in animals. Mol Cell 38(3):323–332CrossRef PubMed
  12.Yeung ML, Benkirane M, Jeang K-T (2007) Small non-coding RNAs, mammalian cells, and viruses: regulatory interactions? Retrovirology 4(1):74PubMedCentral CrossRef PubMed
  13.J-z Sun (2013) Wang J, Yuan D, Wang S, Li Z, Yi B, Mao Y, Hou Q, Liu W. Cellular microRNA miR-181b inhibits replication of mink enteritis virus by repression of non-structural protein 1 translation. PLoS One 8(12):e81515CrossRef
  14.Pillet S, Fichelson S, Morinet F (2008) Human B19 erythrovirus in vitro replication: what’s new? J Virol 82(17):8951–8953PubMedCentral CrossRef PubMed
  15.Wolfisberg R, Ruprecht N, Kempf C, Ros C (2013) Impaired genome encapsidation restricts the in vitro propagation of human parvovirus B19. J Virol Methods 193(1):215–225CrossRef PubMed
  16.Pozzuto T, von Kietzell K, Bock T, Schmidt-Lucke C, Poller W, Zobel T, Lassner D, Zeichhardt H, Weger S, Fechner H (2011) Transactivation of human parvovirus B19 gene expression in endothelial cells by adenoviral helper functions. Virology 411(1):50–64CrossRef PubMed
  17.Chen AY, Guan W, Lou S, Liu Z, Kleiboeker S, Qiu J (2010) Role of erythropoietin receptor signaling in parvovirus B19 replication in human erythroid progenitor cells. J Virol 84:12385–12396PubMedCentral CrossRef PubMed
  18.Luo Y, Lou S, Deng X, Liu Z, Li Y, Kleiboeker S, Qiu J (2011) Parvovirus B19 infection of human primary erythroid progenitor cells triggers ATR-Chk1 signaling, which promotes B19 virus replication. J Virol 85(16):8046–8055PubMedCentral CrossRef PubMed
  19.Chen AY, Kleiboeker S, Qiu J (2011) Productive parvovirus B19 infection of primary human erythroid progenitor cells at hypoxia is regulated by STAT5A and MEK signaling but not HIFα. PLoS Pathog 7(6):e1002088PubMedCentral CrossRef PubMed
  20.Filipowicz W, Bhattacharyya SN, Sonenberg N (2008) Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet 9(2):102–114CrossRef PubMed
  21.Zhang Y, Li Y-k (2013) MicroRNAs in the regulation of immune response against infections. J Zhejiang Univ Sci B 14(1):1–7PubMedCentral CrossRef PubMed
  22.Huang J, Wang F, Argyris E, Chen K, Liang Z, Tian H, Huang W, Squires K, Verlinghieri G, Zhang H (2007) Cellular microRNAs contribute to HIV-1 latency in resting primary CD4 + T lymphocytes. Nat Med 13(10):1241–1247CrossRef PubMed
  23.Chiang K, Rice AP (2012) MicroRNA-mediated restriction of HIV-1 in resting CD4 + T cells and monocytes. Viruses 4(9):1390–1409PubMedCentral CrossRef PubMed
  24.Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136(2):215–233PubMedCentral CrossRef PubMed
  
• 作者单位：Azadeh Anbarlou (1)
  Mahshid AkhavanRahnama (2)
  Amir Atashi (1)
  Masoud Soleimani (1)
  Ehsan Arefian (3)
  Giorgio Gallinella (4)
  
  1. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran
  2. Department of Hematology, Tabriz University of Medical Sciences, Tabriz, Iran
  3. Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  4. Department of Pharmacy and Biotechnology, S.Orsola-Malpighi Hospital - Microbiology, University of Bologna, Bologna, Italy
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

Human Parvovirus B19 (PVB19) is one of the most important pathogens that targets erythroid lineage. Many factors were mentioned for restriction to erythroid progenitor cells (EPCs). Previous studies showed that in non-permissive cells VP1 and VP2 (structural proteins) mRNAs were detected but could not translate to proteins. A bioinformatics study showed that this inhibition might be due to specific microRNAs (miRNAs) present in non-permissive cells but not in permissive EPCs. To confirm the hypothesis, we evaluated the effect of miRNAs on VP expression. CD34+ HSCs were separated from cord blood. Then, CD34+ cells were treated with differentiation medium to obtain CD36+ EPCs. To evaluate the effect of miRNAs on VP expression in MCF7 and HEK-293 cell lines (non-permissive cells) and CD36+ EPCs, dual luciferase assay was performed in presence of shRNAs against Dicer and Drosha to disrupt miRNA biogenesis. QRT-PCR was performed to check down-regulation of Dicer and Drosha after transfection. All measurements were done in triplicate. Data means were compared using one-way ANOVAs. MicroRNA prediction was done by the online microRNA prediction tools. No significant difference was shown in luciferase activity of CD36+ EPCs after co-transfection with shRNAs, while it was significant in non-permissive cells. Our study revealed that miRNAs may be involved in inhibition of VP expression in non-permissive cells, although further studies are required to demonstrate which miRNAs exactly are involved in regulation of PVB19 replication.