Unconventional EGF-induced ERK1/2-mediated Kv1.3 endocytosis

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• 作者：Ramón Martínez-Mármol ; Núria Comes…
• 关键词：Olfactory bulb ; Sensory neurons ; Map kinases ; Tyrosine kinases ; Endocytosis ; Voltage ; dependent potassium channels
• 刊名：Cellular and Molecular Life Sciences (CMLS)
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：73
• 期：7
• 页码：1515-1528
• 全文大小：2,592 KB
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• 作者单位：Ramón Martínez-Mármol (1) (2)
  Núria Comes (1)
  Katarzyna Styrczewska (1)
  Mireia Pérez-Verdaguer (1)
  Rubén Vicente (3)
  Lluís Pujadas (2)
  Eduardo Soriano (2) (4) (5)
  Alexander Sorkin (6)
  Antonio Felipe (1) (7)
  
  1. Molecular Physiology Laboratory, Departament de Bioquímica i Biologia Molecular, Institut de Biomedicina (IBUB), Barcelona, Spain
  2. Departament de Biologia Celular, Universitat de Barcelona, Barcelona, Spain
  3. Laboratory of Molecular Physiology and Channelopathies, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain
  4. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, Spain
  5. Vall d´Hebron Institute of Research (VHIR) and Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain
  6. Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
  7. Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Life Sciences
  Biochemistry
  
• 出版者：Birkh盲user Basel
• ISSN：1420-9071

文摘

The potassium channel Kv1.3 plays roles in immunity, neuronal development and sensory discrimination. Regulation of Kv1.3 by kinase signaling has been studied. In this context, EGF binds to specific receptors (EGFR) and triggers tyrosine kinase-dependent signaling, which down-regulates Kv1.3 currents. We show that Kv1.3 undergoes EGF-dependent endocytosis. This EGF-mediated mechanism is relevant because is involved in adult neural stem cell fate determination. We demonstrated that changes in Kv1.3 subcellular distribution upon EGFR activation were due to Kv1.3 clathrin-dependent endocytosis, which targets the Kv1.3 channels to the lysosomal degradative pathway. Interestingly, our results further revealed that relevant tyrosines and other interacting motifs, such as PDZ and SH3 domains, were not involved in the EGF-dependent Kv1.3 internalization. However, a new, and yet undescribed mechanism, of ERK1/2-mediated threonine phosphorylation is crucial for the EGF-mediated Kv1.3 endocytosis. Our results demonstrate that EGF triggers the down-regulation of Kv1.3 activity and its expression at the cell surface, which is important for the development and migration of adult neural progenitors.