Phylogeography of the Asian rice gall midge Orseolia oryzae (Wood Mason) (Diptera: Cecidomyiidae) in Thailand
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- 作者：Solene Janique ; Wantana Sriratanasak ; Kulchana Ketsuwan ; Jirapong Jairin…
- 关键词：DNA barcoding ; Mitochondrial DNA ; Phylogeography ; Orseolia oryzae ; Repeated motif ; Thailand
- 刊名：Genetica
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：145
- 期：1
- 页码：37-49
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Animal Genetics and Genomics; Plant Genetics & Genomics; Evolutionary Biology; Human Genetics; Microbial Genetics and Genomics;
- 出版者：Springer International Publishing
- ISSN：1573-6857
- 卷排序：145
文摘
The Asian rice gall midge (RGM) Orseolia oryzae (Wood Mason) (Diptera: Cecidomyiidae) is a major pest of rice, leading to yield losses in Thailand and many Asian countries. Despite an increasing number of reported midge outbreaks and the presence of many susceptible rice varieties, only a few studies have focused on the genetic variation of the midges. Therefore, we analyzed the phylogeography among Thai RGM populations covering north, northeast and central Thailand. Two mitochondrial DNA genes, cytochrome C oxidase I (COI) and 12S, and a non-coding repeat region (RR) situated just before COI were amplified. Overall, the haplotype diversity for COI and 12S genes of the Thai population was high, but the nucleotide diversity was quite low. Altogether, the phylogenetic tree and pairwise Fst values indicated that Thai RGM populations recently expanded and were homogeneously distributed throughout the country, except for some populations in the north, which most likely became recently isolated from the main population. Two non-coding repeat motifs, that were recently observed in the mitogenome of RGM in India, were absent in Thai populations and replaced by an 89 bp non-coding sequence. Tandem nucleotide repeats of the sequence TA were also observed. The repeat copy number varied from 2 to 11 and was not correlated with geographical repartition of the midge. Finally, COI barcoding divergence between Indian and Thai populations was high (6.3% in average), giving insights into the potential existence of an RGM species complex in Asia.