RCAS-RNAi: A loss-of-function method for the developing chick retina

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• 作者：Sanjiv Harpavat (1)
  Constance L Cepko (1)
  
• 刊名：BMC Developmental Biology
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：6
• 期：1
• 全文大小：800KB
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• 作者单位：Sanjiv Harpavat (1)
  Constance L Cepko (1)
  
  1. Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston, USA
  

文摘

Background The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. Results We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, the amount of retinal tissue affected can be controlled by manipulating the degree of infection. Conclusion This technique provides a rapid and efficient loss-of-function option for studies in the developing chick retina.