Heme oxygenase-1 and interleukin-11 are overexpressed in stress-induced premature senescence of human WI-38 fibroblasts induced by tert-butylhydroperoxide and ethanol

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• 作者：Thierry Pascal ; Florence Debacq-Chainiaux ; Emmanuelle Boilan ; No?lle Ninane ; Martine Raes and Olivier Toussaint
• 关键词：Cellular senescence ; Oxidative stress ; Ethanol ; Human fibroblasts ; Heme oxygenase ; Interleukin ; 11
• 刊名：Biogerontology
• 出版年：2007
• 出版时间：August, 2007
• 年：2007
• 卷：8
• 期：4
• 页码：409-422
• 全文大小：392 KB

文摘

Acute repeated exposures to subcytotoxic concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of human diploid fibroblasts. In the present work we found an increased mRNA and protein level of interleukin-11 and heme oxygenase-1 in premature senescence of WI-38 human diploid foetal lung fibroblasts induced by both tert-butylhydroperoxide and ethanol. We tested whether interleukin-11 and heme oxygenase-1 could protect against tert-butylhydroperoxide- or ethanol-induced premature senescence when stable overexpression was established using a retroviral vector-based transduction. No protective effect was found against the decrease of the proliferative potential, the increase of the proportion of senescence-associated ?-galactosidase positive cells and the increase of the mRNA levels of six senescence-associated genes.