Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass
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- 作者：Mi Li ; Yunqiao Pu ; Chang Geun Yoo ; Erica Gjersing…
- 关键词：Switchgrass ; Caffeic acid O ; methyltransferase ; Biomass recalcitrance ; Enzymatic hydrolysis ; Lignin ; Cellulose accessibility
- 刊名：Biotechnology for Biofuels
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：10
- 期：1
- 全文大小：1403KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
- 出版者：BioMed Central
- ISSN：1754-6834
- 卷排序：10
文摘
BackgroundThe native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants. We determined the chemical composition, cellulose crystallinity and the degree of its polymerization, molecular weight of hemicellulose, and cellulose accessibility of cell walls in order to better understand the fundamental features of why biomass is recalcitrant to conversion without pretreatment. The most important is to investigate whether traits and features are stable in the dynamics of field environmental effects over multiple years.