Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

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• 作者：Yi-Chun Chang (1) (2)
  Shou-Hsien Li (1)
  Ming-Hsung Chang (2)
  Szu-Lung Chen (2)
  
• 关键词：False gharial ; Gavialidae ; Crocodilian ; Microsatellite
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：6
• 期：2
• 页码：495-498
• 全文大小：
• 参考文献：1. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92-4 CrossRef
  2. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202-22 CrossRef
  3. IUCN (2013) IUCN red list of threatened species, version 2013.2. list.org" class="a-plus-plus">www.iucnredlist.org
  4. Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1006-099 CrossRef
  5. Rousset F (2008) GENEPOP-07: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103-06 CrossRef
  
• 作者单位：Yi-Chun Chang (1) (2)
  Shou-Hsien Li (1)
  Ming-Hsung Chang (2)
  Szu-Lung Chen (2)
  
  1. Department of Life Science, National Taiwan Normal University, Taipei, Taiwan, ROC
  2. Conservation and Research Center, Taipei Zoo, 30, Xin-Guang Rd, Sec 2, Taipei, 116, Taiwan, ROC
  
• ISSN：1877-7260

文摘

Tomistoma schlegelii is an endangered crocodilian with an adult population size less than 2,500 individuals. To make appropriate in situ and ex situ conservation strategies, it is essential to perform parentage assignment for breeding captive populations and to conduct population study in wild populations based on genetic data. However, there is no suitable genetic marker available for T. schlegelii. Here, we reported 18 novel microsatellite loci from the tomistoma. Allele numbers of these loci ranged from 2 to 16, and their observed heterozygosity ranged from 0.105 to 0.684. The probability for excluding false parental pairs using the 18 loci was 99.9999?%. These markers are useful not only in parentage assignment but also in population structure analysis.