Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS)
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- 作者：Kimberly Kaplan (1)
Shelley Jackson (2)
Prabha Dwivedi (1)
W. Sean Davidson (3)
Qing Yang (3)
Patrick Tso (3)
William Siems (1)
Amina Woods (2)
Herbert H. Hill Jr. (1) - 关键词：MALDI ; IMMS ; Metabolomics ; Principal component analysis
- 刊名：International Journal for Ion Mobility Spectrometry
- 出版年：2013
- 出版时间：September 2013
- 年：2013
- 卷：16
- 期：3
- 页码：177-184
- 全文大小：584KB
- 参考文献：1. Shroff R, Rulisek L, Doubsky J, Svatos A (2009) Acid-base-driven matrix-assisted mass spectrometry for targeted metabolomics. PNAS 106:10092-0096 CrossRef
2. Bedair M, Sumner LW (2008) Current and emerging mass-spectrometry technologies for metabolomics. TRAC 27:238-50
3. Want EJ, Nordstrom A, Morita H, Siuzdak G (2007) From exogenous to endogenous: The inevitable imprint of mass spectrometry in metabolomics. J Proteome Research 6:459-68 CrossRef
4. Ruotolo BT, Gillig KJ, Woods AS, Egan TF, Ugarov MV, Schultz JA, Russell DH (2004) Analysis of Phosphorylated Peptides by Ion Mobility-Mass Spectrometry. Anal Chem 76:6727-733 CrossRef
5. Vaidyanathan S, Gaskell S, Goodacre R (2006) Matrix-suppressed laser desorption/ionisation mass spectrometry and its suitability for metabolome analyses. Rapid Commun Mass Spectrom 20:1192-198 CrossRef
6. Shen Z, Thomas JJ, Averbuj C, Broo KM, Engelhard M, Crowell JE, Finn MG, Siuzdak G (2001) Porous silicon as a versatile platform for laser desorption/ionization mass spectrometry. Anal Chem 73:612-19 CrossRef
7. Kanu AB, Dwivedi P, Tam M, Matz L, Hill HH (2008) Ion mobility–mass spectrometry. J of Mass Spec 43:1-2 CrossRef
8. Dwivedi P, Puzon G, Tam M, Langlais D, Jackson S, Kaplan K, Siems WF, Schultz AJ, Xun L, Woods A, Hill HH (2010) Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source. Journal of Mass Spec 45:1383-393 CrossRef
9. Jackson SN, Ugarov M, Egan T, Post JD, Langlais D, Schultz JA, Woods AS (2007) MALDI-ion mobility-TOFMS imaging of lipids in rat brain tissue. Journal of Mass Spec 42:1093-098 CrossRef
10. Woods AS, Koomen JM, Ruotolo BT, Gillig KJ, Russel DH, Fuhrer K, Gonin M, Egan TF, Schultz JA (2002) A study of peptide–peptide interactionsusing MALDI ion mobility o-TOF and ESImass spectrometry. Journal of the American Society for Mass Spec 13:166-69 CrossRef
11. Woods AS, Fuhrer K, Gonin M, Egan T, Ugarov M, Gillig KJ, Schultz JA (2003) Angiotensin II–Acetylcholine Noncovalent Complexes Analyzed With MALDI–Ion Mobility–TOF MS. J Biomol Tech 14:1-
12. Clowers BH, Dwivedi P, Steiner WE, Hill HH, Bendiak B (2005) Separation of Sodiated Isobaric Disaccharides and Trisaccharides Using Electrospray Ionization-Atmospheric Pressure Ion Mobility-Time of Flight Mass Spectrometry. Journal of the American Society for Mass Spec 16:660-69 CrossRef
13. Williams JP, Bugarcic T, Habtemariam A, Giles K, Campuzano I, Rodger PM, Sadler PJ (2009) Isomer Separation and Gas-Phase Configurations of Organoruthenium Anticancer Complexes: Ion Mobility Mass Spectrometry and Modeling. Journal of the American Society for Mass Spec 20:1119-122 CrossRef
14. Wu C, Siems WF, Klasmeier J, Hill HH (2000) Separation of Isomeric Peptides Using Electrospray Ionization/High-Resolution Ion Mobility Spectrometry. Anal Chem 72:391-95 CrossRef
15. Asbury GR, Hill HH (2000) Separation of amino acids by ion mobility spectrometry. J of Chrom A 902:433-37 CrossRef
16. Jackson SN, Wang HYJ, Ugarov M, Egan T, Schultz JA, Woods AS (2005) Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS. J of the American Society for Mass Spec 16:133-38 CrossRef
17. Kaplan K, Dwivedi P, Davidson S, Yang Q, Tso P, Siems W, Hill HH (2009) Monitoring Dynamic Changes in Lymph Metabolome of Fasting and Fed Rats by Electrospray Ionization-Ion Mobility Mass Spectrometry (ESI-IMMS). Anal Chem 81:7944-953 CrossRef
18. Karasek FW (1971) Plasma chromatography of the polychlorinated biphenyls. Anal Chem 43:1982-986 CrossRef
19. McLean JA (2009) The Mass-Mobility Correlation Redux: The Conformational Landscape of Anhydrous Biomolecules. J of the American Society for Mass Spec 20:1775-781 CrossRef
20. Woods AS, Ugarov M, Egan T, Koomen J, Gillig KJ, Fuhrer K, Gonin M, Schultz JA (2004) Lipid/Peptide/Nucleotide Separation with MALDI-Ion Mobility-TOF MS. Anal Chem 76:2187-195 CrossRef
21. Zhang XW, Yap YL, Wei D, Chen G, Chen F (2008) Novel omics technologies in nutrition research. Biotechnol Adv 26:169-76 CrossRef
22. Gibney MJ, Walsh M, Brennan L, Roche HM, German B, Van Ommen B (2005) Metabolomics in human nutrition: Opportunities and challenges. American J of Clinical Nutrition 82:497-03
23. Fardet A, Llorach R, Martin JF, Besson C, Lyan B, Pujos-Guillot E, Scalbert A (2008) A Liquid Chromatography?Quadrupole Time-of-Flight (LC?QTOF)-based Metabolomic Approach Reveals New Metabolic Effects of Catechin in Rats Fed High-Fat Diets. Journal of Proteome Research 7:2388-398 CrossRef
24. Gu HW, Chen HW, Pan ZZ, Jackson AU, Talaty N, Xi BW, Kissinger C, Duda C, Mann D, Raftery D, Cooks RG (2007) Monitoring Diet Effects via Biofluids and Their Implications for Metabolomics Studies. Anal Chem 79:89-7 CrossRef
25. Crews B, Wikoff WR, Patti GJ, Woo HK, Kalisiak E, Heideker J, Siuzdak G (2009) Variability Analysis of Human Plasma and Cerebral Spinal Fluid Reveals Statistical Significance of Changes in Mass Spectrometry-Based Metabolomics Data. Anal Chem 81:8538-544 CrossRef
26. Jolliffe IT (2002) Principal Component Analysis, 2nd edn. Springer-Verlag New York, Inc, New York
27. Bollman JL, Cain JC, Grindlay JH (1948) Techniques for the collection of lymph from the liver, small intestine, or thoracic duct of the rat. The J of Laboratory and Clinical Medicine 33:1349-352
28. Tso P, Balint JA, Bishop MB, Rodgers JB (1981) Acute inhibition of intestinal lipid transport by Pluronic L-81 in the rat. Am J Physiol Gastrointest Liver Physiol 41:G487–G497
29. Chen IS, Shen CSJ, Sheppard A (1981) Comparison of methylene chloride and chloroform for the extraction of fats from food products. J Journal of the American Oil Chemists Society 58:599-01 CrossRef
30. Wishard DS, Tzur D, Knox C, Eisner R, ChiGuo A, Young N et al (2007) HMDB: The Human Metabolome Database. Nucleic Acids Res 35:D521–D526 CrossRef - 作者单位：Kimberly Kaplan (1)
Shelley Jackson (2)
Prabha Dwivedi (1)
W. Sean Davidson (3)
Qing Yang (3)
Patrick Tso (3)
William Siems (1)
Amina Woods (2)
Herbert H. Hill Jr. (1)
1. Washington State University, Pullman, WA, 99164, USA
2. NIDA IRP, NIH, Baltimore, MD, 21224, USA
3. University of Cincinnati, Cincinnati, OH, 45221, USA
文摘
Matrix-assisted laser/desorption ionization (MALDI) time-of-flight mass spectrometry (TOF) has been investigated for use in the field of metabolomics; however, difficulties, mainly due to chemical interferences, are typically encountered. By coupling MALDI with ion mobility time-of-flight mass spectrometry (IMMS), isomers and isobars are resolved in mobility space reducing the chemical interference from matrix/background ions. MALDI-IMMS offers the advantages of high sensitivity, high throughput and low sample consumption. For this study, MALDI-IMMS was evaluated by monitoring metabolic changes in lymphatic fluid collected from fasting and fed rats. The number of metabolite features detected in the samples ranged between 1200 and 3400 depending on the duration between the feeding time and lymph sample collection. There were 747 metabolite features that were statistically analyzed by principal component analysis (PCA). From the 3-D score plots of PC1, PC2 and PC3 65?% of the original variation of the system was explained and the differences between the samples were demonstrated.