Uniform design for optimizing biomass and intracellular polysaccharide production from self-flocculating Scenedesmus sp.-BH

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• 作者：Yingying He (1)
  Lin Zhou (1)
  Xiaolin Xu (1) (3)
  Siyu Wang (1)
  Changhai Wang (2)
  Bin Dai (1) (3)
  
• 关键词：Scenedesmus sp. ; BH ; Medium components ; Uniform design ; Biomass ; Intercellular polysaccharides
• 刊名：Annals of Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：64
• 期：4
• 页码：1779-1787
• 全文大小：1,211 KB
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• 作者单位：Yingying He (1)
  Lin Zhou (1)
  Xiaolin Xu (1) (3)
  Siyu Wang (1)
  Changhai Wang (2)
  Bin Dai (1) (3)
  
  1. School of Chemistry and Chemical Engineering, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, Xinjiang, China
  3. School of Chemistry and Chemical Engineering, Shihezi University, North Quad Road, Shihezi, 832003, Xinjiang, China
  2. College of Resources & Environmental Science, Nanjing Agricultural University, Nanjing, 210095, China
  
• ISSN：1869-2044

文摘

Four microalgae species were isolated from freshwaters in Xinjiang, one of which was identified as Scenedesmus sp.-BH. Analysis of the specimens showed not only the characterization of self-flocculation with a flocculating rate of 92.29?% in 12?h, but relatively high biomass and intracellular polysaccharide (IPS) production (250.5 and 5.55?mg/L) under original BG11 medium. Uniform design was applied to optimize the nutritional conditions of Scenedesmus sp.-BH. The maximum biomass yield of 556.7?±-6.97?mg/L appeared at NaNO3 0.77?g/L, K2HPO4·3H2O 0.24?g/L, MgSO4·7H2O 0.24?g/L, ferric ammonium citrate 0.021?g/L, and NaHCO3 3.85?g/L, while a maximum IPS yield of 14.3?±-.46?mg/L appeared at NaNO3 0.004?g/L, K2HPO4·3H2O 0.24?g/L, MgSO4·7H2O 0.21?g/L, ferric ammonium citrate 0.024?g/L, and NaHCO3 1.42?g/L. Both biomass and IPS production were over two-fold of those cultured under the original condition, which demonstrated that uniform design could successfully improve the production of biomass and IPS.