Production and Characteristics of a Bioflocculant by Klebsiella pneumoniae YZ-6 Isolated from Human Saliva

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• 作者：Zhengshan Luo (1)
  Li Chen (2)
  Changhong Chen (1)
  Wei Zhang (1)
  Ming Liu (1)
  Ye Han (1)
  Jiangang Zhou (1)
  
• 关键词：Klebsiella pneumoniae ; Bioflocculant ; Thermostable ; Polysaccharide
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：172
• 期：3
• 页码：1282-1292
• 全文大小：446 KB
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• 作者单位：Zhengshan Luo (1)
  Li Chen (2)
  Changhong Chen (1)
  Wei Zhang (1)
  Ming Liu (1)
  Ye Han (1)
  Jiangang Zhou (1)
  
  1. School of Environmental Engineering, Wuhan Textile University, FangZhi Road, Wuhan, 430073, China
  2. Central China Normal University Library, Wuhan, 430079, China
  
• ISSN：1559-0291

文摘

The production and characterization of a bioflocculant, MBF-6, by Klebsiella pneumoniae was investigated. Optimum culture conditions for bioflocculant production were an initial medium pH of 7, an incubation temperature of 30?°C, and an inoculum size of 1?% (v/v) of cell density 1.0?×-08?cfu/mL. The carbon, nitrogen, and cation sources for optimum bioflocculant production were glucose, peptone, and ZnCl2. The bioflocculant mainly consisted of protein (3.4?%) and sugar (95.1?%). Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl and hydroxyl groups while the thermogravimetric analysis (TGA) showed a degradation temperature (T d) of 81.4?°C. MBF-6 had a good flocculating rate in kaolin suspension without cation addition and was stable over a wide range of pH and temperature. Investigation on the flocculation efficacy of the characterized MBF-6 for wastewater treatment of dairy, woolen, brewery, and sugar industries suggested it to be effective.