Genome-wide identification and characterization of tRNA-derived RNA fragments in land plants
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- 作者：Cristiane S. Alves ; Renato Vicentini ; Gustavo T. Duarte…
- 关键词：Small RNA ; tRNA ; derived RNA fragment ; tRF ; Arabidopsis thaliana ; High throughput data
- 刊名：Plant Molecular Biology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：93
- 期：1-2
- 页码：35-48
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences; Biochemistry, general; Plant Pathology;
- 出版者：Springer Netherlands
- ISSN：1573-5028
- 卷排序：93
文摘
Key messageThe manuscript by Alves et al. entitled “Genome-wide identification and characterization of tRNA-derived RNA fragments in land plants” describes the identification and characterization of tRNAderived sRNA fragments in plants. By combining bioinformatic analysis and genetic and molecular approaches, we show that tRF biogenesis does not rely on canonical microRNA/siRNA processing machinery (i.e., independent of DICER-LIKE proteins). Moreover, we provide evidences that the Arabidopsis S-like Ribonuclease 1 (RNS1) might be involved in the biogenesis of tRFs. Detailed analyses showed that plant tRFs are sorted into different types of ARGONAUTE proteins and that they have potential target candidate genes. Our work advances the understanding of the tRF biology in plants by providing evidences that plant and animal tRFs shared common features and raising the hypothesis that an interplay between tRFs and other sRNAs might be important to fine-tune gene expression and protein biosynthesis in plant cells.