Bilirubin clearance and antioxidant activities of ethanol extract of Phyllanthus amarus root in phenylhydrazine-induced neonatal jaundice in mice

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• 作者：Soumya Maity (1)
  Nivedita Nag (1)
  Suchandra Chatterjee (2)
  Soumyakanti Adhikari (3)
  Santasree Mazumder (1)
  
• 关键词：Neonatal jaundice ; Oxidative stress ; Phyllanthus amarus ; Bilirubin ; lowering effect ; CAR ; CYP3A1
• 刊名：Journal of Physiology and Biochemistry
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：69
• 期：3
• 页码：467-476
• 全文大小：407KB
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• 作者单位：Soumya Maity (1)
  Nivedita Nag (1)
  Suchandra Chatterjee (2)
  Soumyakanti Adhikari (3)
  Santasree Mazumder (1)
  
  1. Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India
  2. Food Technology Division, Bhabha Atomic Research Centre, Mumbai, 400085, India
  3. Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, India
  
• ISSN：1877-8755

文摘

The ability of ethanol extract of Phyllanthus amarus root (EEPA) to decrease bilirubin level and oxidative stress in phenylhydrazine-induced neonatal jaundice in mice was investigated. Administration of phenylhydrazine (75?mg/kg b.w.) significantly elevated total and unconjugated serum bilirubin level compared to control mice. EEPA (5, 10, and 20?mg/kg b.w., oral) dose-dependently reduced the bilirubin level. EEPA treatment also upregulated hepatic CAR and CYP3A1, accounting for its ability to facilitate bilirubin clearance. A single dose of EEPA (20?mg/kg b.w.) induced higher level of bilirubin clearance than phototherapy, widely used for treating neonatal jaundice. Furthermore, phenylhydrazine administration significantly increased MDA, protein carbonyl, and total thiol content and lowered the GSH level along with superoxide dismutase and catalase activity in erythrocyte compared to the control group. Single administration of EEPA (20?mg/kg b.w.) significantly reversed the trend. Presence of gallic acid, gentisic acid, and ortho-coumaric acid in EEPA was identified by HPLC analysis. Amongst these, the major phenolic constituent, gallic acid, exhibited significant bilirubin-lowering effect. These results suggested that P. amarus may be beneficial in reducing bilirubin level as well as oxidative stress in neonatal jaundice.