p53 activation and mitochondria-mediated pathway are involved during hanging death-induced neuronal cell apoptosis in dentate gyrus region of the rat brain

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• 作者：Sabana Khatun ; Shail K Chaube ; Chandra N Bhattacharyya
• 关键词：Forensic science ; Hanging death ; p53 ; Cytochrome c ; Caspases activity ; Apoptosis ; Necrosis ; Neuronal cells
• 刊名：SpringerPlus
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：2
• 期：1
• 全文大小：2095KB
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• 作者单位：Sabana Khatun (3)
  Shail K Chaube (3)
  Chandra N Bhattacharyya (3)
  
  3. Cell Physiology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221005, India
  
• 刊物类别：Science, general;
• 刊物主题：Science, general;
• 出版者：Springer International Publishing
• ISSN：2193-1801

文摘

The goal of this study was to understand the molecular event in the brain caused by hanging death (HD). Animals were subjected to either cervical dislocation (CD) or HD. Brain was collected at various times (0, 1, 3, 6 and 12 h) after death. Brain expression of p53 and Bax, cytochrome c concentration, caspases activity and DNA fragmentation were analyzed. Compared to that of CD, HD increased p53 and Bax proteins expressions, cytochrome c concentration, caspases activity and DNA fragmentation during the early period (0鈥? h) of HD, whereas CD induced necrosis 3 h post- CD and thereafter. These data support that HD induces neuronal cell apoptosis, in part, through mitochondria-mediated pathways. These data also suggest that neuronal apoptosis could be a potential marker and an aid to forensic science of HD. Keywords Forensic science Hanging death p53 Cytochrome c Caspases activity Apoptosis Necrosis Neuronal cells