Characterization of Gag and Nef-specific ELISpot-based CTL responses in HIV-1 infected Indian individuals

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• 作者：Sanjay Mendiratta ; Madhu Vajpayee ; Uma Malhotra ; Shweta Kaushik ; Lalit Dar ; Kamalika Mojumdar ; Neeraj Kumar Chauhan and Vishnubhatla Sreenivas
• 关键词：HIV ; 1 specific ; ELISpot ; Gag ; Nef ; CTL responses ; India
• 刊名：Medical Microbiology and Immunology
• 出版年：2009
• 出版时间：February, 2009
• 年：2009
• 卷：198
• 期：1
• 页码：47-56
• 全文大小：424.0 KB

文摘

Cytotoxic T lymphocyte (CTL) responses to Gag have been most frequently linked to control of viremia whereas CTL responses to Nef have direct relationship with viral load. IFN-γ ELISpot assay was used to screen CTL responses at single peptide level directed at HIV-1 subtype C Gag and Nef proteins in 30 antiretroviral therapy naive HIV-1 infected Indian individuals. PBMCs from 73.3 % and 90 % of the study population showed response to Gag and Nef antigens, respectively. The magnitude of Gag-specific CTL responses was inversely correlated with plasma viral load (r = −0.45, P = 0.001), whereas magnitude of Nef-specific responses was directly correlated (r = 0.115). Thirteen immunodominant regions (6 in Gag, 7 in Nef) were identified in the current study. The identification of Gag and Nef-specific responses across HIV-1 infected Indian population and targeting epitopes from multiple immunodominant regions may provide useful insight into the designing of new immunotherapy and vaccines.