Characterization and enzymatic hydrolysis of hydrothermally treated β-1,3–1,6-glucan from Aureobasidium pullulans

详细信息    查看全文

• 作者：Katsuki Hirabayashi ; Nobuhiro Kondo…
• 关键词：β ; 1 ; 3–1 ; 6 ; Glucan ; Aureobasidium pullulans ; Hydrothermal treatment ; Chemical structure ; Enzymatic hydrolysis
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：32
• 期：12
• 全文大小：931 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972
• 卷排序：32

文摘

The chemical structure of hydrothermally treated β-1,3–1,6-glucan from Aureobasidium pullulans was characterized using techniques such as gas chromatography/mass spectrometry (GC/MS) and nuclear magnetic resonance (NMR). The chemical shifts of anomeric carbons observed in the 13C-NMR spectra suggested the presence of single flexible chains of polysaccharide in the sample. β-1,3–1,6-Glucan from A. pullulans became water-soluble, with an average molecular weight of 128,000 Da after hydrothermal treatment, and the solubility in water was approximately 10% (w/w). Sample (3% w/v) was completely hydrolyzed to glucose by enzymatic reaction with Lysing enzymes from Trichoderma harzianum. Gentiobiose (Glcβ1 → 6Glc) and glucose were released as products during the reaction, and the maximum yield of gentiobiose was approximately 70% (w/w). The molar ratio of gentiobiose to glucose after 1 h reaction suggested that the sample is likely highly branched. Sample (3% w/v) was also hydrolyzed to glucose by Uskizyme from Trichoderma sp., indicating that it is very sensitive to enzymatic hydrolysis.