Novel interaction between CCR4 and CAF1 in rice CCR4–NOT deadenylase complex
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- 作者：Wei-Lun Chou ; Yue-Lin Chung ; Jhen-Cheng Fang ; Chung-An Lu
- 关键词：Carbon catabolite repressor 4 ; CCR4 ; associated factor 1 ; CCR4–NOT complex ; Deadenylation ; Poly(A) tail ; Rice ; Oryza sativa
- 刊名：Plant Molecular Biology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：93
- 期：1-2
- 页码：79-96
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences; Biochemistry, general; Plant Pathology;
- 出版者：Springer Netherlands
- ISSN：1573-5028
- 卷排序：93
文摘
Key messageRice is an important crop in the world. However, little is known about rice mRNA deadenylation, which is an important regulation step of gene expression at the post-transcriptional level. The CCR4–NOT1 complex contains two key components, CCR4 and CAF1, which are the main cytoplasmic deadenylases in eukaryotic cells. In yeast and humans, CCR4 can interact with CAF1 via its N-terminal LRR domain. However, no CCR4 protein containing N-terminal LRR motifs have been found in plants. In this manuscript, we demonstrate a novel pattern of interaction between OsCCR4 and OsCAF1 in the rice CCR4–NOT complex, and that OsCAF1 acts as a bridge between OsCCR4 and OsNOT1 in this complex. Our results revealed that the Mynd-like domain at the N-terminus of rice CCR4 proteins and the PXLXP motif at the rice CAF1 N-terminus play critical roles in OsCCR4–OsCAF1 interaction.