A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters
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- 作者：Hai-Bo Wang ; Ying Chen ; Na Li ; Yan-Ming Liu
- 关键词：Fluorescent bioassay ; Nanoprobes ; Glucose detection ; Glucose oxidase ; Enzymatically generated H2O2 ; Glucuronic acid ; Mn(II) ions
- 刊名：Microchimica Acta
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：184
- 期：2
- 页码：515-523
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
- 出版者：Springer Vienna
- ISSN：1436-5073
- 卷排序：184
文摘
The authors describe a fluorometric glucose assay that is based on the use of MnO2 nanosheets and copper nanoclusters (CuNCs) acting as nanoprobes. The CuNCs were synthesized by using bovine serum albumin as a template by chemical reduction of copper(II) sulfate. On addition of MnO2 nanosheets to a colloidal solution of CuNCs, the fluorescence of CuNCs (measured at excitation/emission wavelengths of 335/410 nm) is quenched. However, in the presence of enzymatically generated H2O2, the MnO2 nanosheets are reduced to form Mn(II) ions. As a result, fluorescence intensity recovers. The glucose assay is based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2 and glucuronic acid. The calibration plot is linear in the 1 μM to 200 μM glucose concentration range, and the detection limit is 100 nM. The method was successfully applied to the determination of glucose in spiked human serum samples.