E2A suppresses invasion and migration by targeting YAP in colorectal cancer cells

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• 作者：Hongchao Zhao (1) (2)
  Ao Huang (1) (2)
  Pu Li (1)
  Yingjun Quan (1) (2)
  Bo Feng (1) (2)
  Xuehua Chen (1)
  Zhihai Mao (1) (2)
  Zhenggang Zhu (1)
  Minhua Zheng (1) (2)
  
• 关键词：Metastasis ; E2A ; Colorectal cancer ; YAP ; EMT
• 刊名：Journal of Translational Medicine
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：11
• 期：1
• 全文大小：1,184 KB
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• 作者单位：Hongchao Zhao (1) (2)
  Ao Huang (1) (2)
  Pu Li (1)
  Yingjun Quan (1) (2)
  Bo Feng (1) (2)
  Xuehua Chen (1)
  Zhihai Mao (1) (2)
  Zhenggang Zhu (1)
  Minhua Zheng (1) (2)
  
  1. Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, 197 Rui Jin Er Rd, Shanghai, 200025, People’s Republic of China
  2. Shanghai Minimally Invasive Surgery Center, Shanghai, China
  
• ISSN：1479-5876

文摘

Background E2A gene, which encodes two basic helix–loop–helix (bHLH) transcription factors E12 and E47, has been identified as regulator of B lymphoid hematopoiesis and suppressor of lymphoma. E47 protein was found to decrease E-cadherin expression and induce epithelial-mesenchymal transition (EMT). However, the role of E2A in colorectal cancer (CRC) metastasis is still elusive. Methods qRT-PCR and semi-qRT-PCR were performed to determine mRNA level of E2A in CRC specimens and colorectal cancer cells. RNAi was employed to downregulate E2A expression and subsequent protein level change was evaluated by immunoblot. Cell invasion and migration capacity were detected by transwell assay using cell culture inserts with or without basement membrane matrix, respectively. Results E2A expression was decreased in metastatic CRC tissues. Invasion and migration assays showed downregulation of E2A increased metastatic capacity of CRC cells while forced expression of E12 or E47 could offset this effect. Both E12 and E47 suppressed EMT induced by E2A downregulation. Moreover, Yes-Associated Protein (YAP) was a downstream target of E2A and suppression of YAP inhibited the pro-migration/invasion of E2A deficiency. Conclusion Our results suggest that E2A suppresses CRC cell metastasis, at least partially if not all, by inhibiting YAP expression.