A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies

详细信息    查看全文

• 作者：Hao Yang (1)
  Qing Guo (1)
  Rong He (1)
  Ding Li (2)
  Xueqing Zhang (1)
  Chenchen Bao (1)
  Hengyao Hu (1)
  Daxiang Cui (1)
  
• 关键词：Antibody ; Diagnosis ; Microarray ; Quantum dots ; Serology
• 刊名：Nanoscale Research Letters
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：4
• 期：12
• 页码：1469-1474
• 全文大小：302KB
• 参考文献：1. L.J. van ‘t Veer, H. Dai, M.J. van de Vijver, Y.D. He, A.A.M. Hart, M. Mao, H.L. Peterse, K. van der Kooy, M.J. Marton, A.T. Witteveen, G.J. Schreiber, R.M. Kerkhoven, C. Roberts, P.S. Linsley, R. Bernards, S.H. Friend, Nature. 415, 530 (2002). doi:10.1038/415530a CrossRef
  2. H. Inoue, A. Matsuyama, K. Mimori, H. Ueo, M. Mori, J.M. Chong, M. Fukayama, T. Kodama, H. Aburatani, Clin. Cancer Res. 8, 3475 (2002)
  3. H.-Y. Wang, D. Greenawalt, X. Cui, I.V. Tereshchenko, M. Luo, Q. Yang, M.A. Azaro, G. Hu, Y. Chu, J.Y. Li, L. Shen, Y. Lin, L. Zhang, H. Li, J. Carcinog. 8, 6 (2009). doi:10.4103/1477-3163.50886 CrossRef
  4. S.L. Volchenboum, C. Li, S. Li, E.F. Attiyeh, C. Patrick Reynolds, J.M. Maris, A. Thomas Look, R.E. George, Cancer Res. 69, 4143 (2009). doi:10.1158/0008-5472 CrossRef
  5. W. Du, X. Ma, D. Nyman, K. Povlsen, N. Akguen, E.M. Schneider, Diagn. Microbiol. Infect. Dis. 59, 355 (2007). doi:10.1016/j.diagmicrobio.2007.06.006 CrossRef
  6. B. Li, D. Zhou, Z. Wang, Z. Song, H. Wang, M. Li, X. Dong, M. Wu, Z. Guo, R. Yang, Microbes Infect. 10, 45 (2008). doi:10.1016/j.micinf.2007.10.003 CrossRef
  7. J. Ingvarsson, C. Wingren, A. Carlsson, P. Ellmark, B. Wahren, G. Engstr?m, U. Harmenberg, M. Krogh, C. Peterson, C.A. Borrebaeck, Proteomics. 8, 2211 (2008). doi:10.1002/pmic.200701167 CrossRef
  8. G.J. Silverman, R. Srikrishnan, K. Germar, C.S. Goodyear, K.A. Andrews, E.M. Ginzler, B.P. Tsao, Clin. Exp. Immunol. 153, 102 (2008). doi:10.1111/j.1365-2249.2008.03680.x CrossRef
  9. B. Pan, L. Ao, F. Gao, H. Tian, R. He, D. Cui, Nanotechnology. 16, 1776 (2005). doi:10.1088/0957-4484/16/9/061 CrossRef
  10. N.-L. Rosi, D.-A. Giljohann, C.-S. Thaxton, A.-K.-R. Lytton-Jean, M.-S. Han, C.-A. Mirkin, Science. 312, 1027 (2006). doi:10.1126/science.1125559 CrossRef
  11. P. Alivisatos, Nat. Biotechnol. 22, 47 (2004). doi:10.1038/nbt927 CrossRef
  12. H.M.E. Azzazy, M.M.H. Mansour, Clin. Chim. Acta. 403, 1 (2009). doi:10.1016/j.cca.2009.01.016 CrossRef
  13. D. Cui, B. Pan, H. Zhang, F. Gao, R. Wu, J. Wang, R. He, T. Asahi, Anal. Chem. 21, 7996 (2008). doi:10.1021/ac800992m CrossRef
  14. J. Drbohlavova, V. Adam, R. Kizek, J. Hubalek, Int. J. Mol. Sci. 10, 656 (2009). doi:10.3390/ijms10020656 CrossRef
  15. I.L. Medintz, H.T. Uyeda, E.R. Goldman, H. Mattoussi, Nat. Mater. 4, 435 (2005). doi:10.1038/nmat1390 CrossRef
  16. M. Han, X. Gao, J.Z. Su, S. Nie, Nat. Biotechnol. 19, 631 (2001). doi:10.1038/90228 CrossRef
  17. E.A. Jares-Erijman, T.M. Jovin, Nat. Biotechnol. 21, 1387 (2003). doi:10.1038/nbt896 CrossRef
  18. X.Y. Huang, L. Li, H.F. Qian, C.Q. Dong, C.J. Ren, Angew. Chem. Int. Ed. 45, 5140 (2006). doi:10.1002/anie.200601196 CrossRef
  19. W.C. Chan, S.M. Nie, Science. 281, 2016 (1998). doi:10.1126/science.281.5385.2016 CrossRef
  20. C.Q. Dong, H.F. Qian, N.H. Fang, J.C. Ren, J. Phys. Chem. B. 110, 11069 (2006). doi:10.1021/jp060279r CrossRef
  21. Y. Chen, X. Qian, Z. Tang, H.D. Banta, F. Hu, J. Cao, J. Huang, Q. Wang, J. Lv, X. Ying, J. Chen, Int. J. Technol. Assess. Health Care. 20, 524 (2004). doi:10.1017/S026646230400145X CrossRef
  22. D. Li, H. Yang, W.H. Zhang, H. Pan, D.Q. Wen, F.C. Han, H.F. Guo, X.M. Wang, X.J. Yan, Gynecol. Obstet. Invest. 62, 220 (2006). doi:10.1159/000094092 CrossRef
  23. J.R. Lakowica, / Effect of Solvents on Fluorescence Emission Spectra, In Principles of Fluorescence Spectroscopy (PIenum Press, New York, 1983), p. 187
  24. A. Sensini, Clin. Microbiol. Infect. 12, 504 (2006). doi:10.1111/j.1469-0691.2006.01444.x CrossRef
  25. E. Mendelson, Y. Aboudy, Z. Smetana, M. Tepperberg, Z. Grossman, Reprod. Toxicol. 21, 350 (2006). doi:10.1016/j.reprotox.2006.02.001 CrossRef
  
• 作者单位：Hao Yang (1)
  Qing Guo (1)
  Rong He (1)
  Ding Li (2)
  Xueqing Zhang (1)
  Chenchen Bao (1)
  Hengyao Hu (1)
  Daxiang Cui (1)
  
  1. Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education,?National Key Laboratory of Micro/Nano Fabrication Technology,Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, 200240, Shanghai, People’s Republic of China
  2. Center of Biological Diagnosis and Therapy, No. 261 Hospital of PLA, 100094, Beijing, China
  
• ISSN：1556-276X

文摘

Quantum dot is a special kind of nanomaterial composed of periodic groups of II–VI, III–V or IV–VI materials. Their high quantum yield, broad absorption with narrow photoluminescence spectra and high resistance to photobleaching, make them become a promising labeling substance in biological analysis. Here, we report a quick and parallel analytical method based on quantum dots for ToRCH-related antibodies including Toxoplasma gondii, Rubella virus, Cytomegalovirus and Herpes simplex virus type 1 (HSV1) and 2 (HSV2). Firstly, we fabricated the microarrays with the five kinds of ToRCH-related antigens and used CdTe quantum dots to label secondary antibody and then analyzed 100 specimens of randomly selected clinical sera from obstetric outpatients. The currently prevalent enzyme-linked immunosorbent assay (ELISA) kits were considered as “golden standard-for comparison. The results show that the quantum dots labeling-based ToRCH microarrays have comparable sensitivity and specificity with ELISA. Besides, the microarrays hold distinct advantages over ELISA test format in detection time, cost, operation and signal stability. Validated by the clinical assay, our quantum dots-based ToRCH microarrays have great potential in the detection of ToRCH-related pathogens.